Williams D E, Park L S
Immunex Research and Development Corporation, Seattle, WA 98101.
Cancer. 1991 May 15;67(10 Suppl):2705-7. doi: 10.1002/1097-0142(19910515)67:10+<2705::aid-cncr2820671706>3.0.co;2-m.
The common functional characteristics of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) may be explained by the presence of a subpopulation of cell surface receptors capable of binding both growth hormones. A GM-CSF/IL-3 fusion protein (pIXY 321) was produced in a yeast expression host. Receptor binding studies with HL-60, JM-1, AML-193, and KG-1 cell lines suggested that the GM-CSF and IL-3 regions had adopted a native conformation within the fusion protein. The fusion protein also exhibited enhanced biologic activity compared with GM-CSF or IL-3 in assays of normal, primary human hematopoietic progenitor cells. pIXY 321 may offer significant clinical advantages over the individual cytokines.
粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-3（IL-3）的共同功能特性可能是由于存在能够结合这两种生长激素的细胞表面受体亚群来解释的。一种GM-CSF/IL-3融合蛋白（pIXY 321）在酵母表达宿主中产生。对HL-60、JM-1、AML-193和KG-1细胞系进行的受体结合研究表明，GM-CSF和IL-3区域在融合蛋白中呈现天然构象。在对正常原代人造血祖细胞的检测中，该融合蛋白与GM-CSF或IL-3相比还表现出增强的生物活性。与单个细胞因子相比，pIXY 321可能具有显著的临床优势。
