Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
Biosci Rep. 2010 Feb 17;30(3):201-7. doi: 10.1042/BSR20090158.
Special codes are embedded in the primary sequence of newly synthesized proteins to determine their final destination. Protein translocation across biological membranes requires co-operation between the targeting and translocation machineries. A conserved membrane channel, the Sec61/SecY complex, mediates protein translocation across or integration into the endoplasmic reticulum membrane in eukaryotes and the plasma membrane in prokaryotes. A combination of recent biochemical and structural data provides novel insights into the mechanism of how the channel allows polypeptide movement into the exoplasmic space and the lipid bilayer.
新合成蛋白质的一级序列中嵌入了特殊的密码子，以确定其最终去向。蛋白质穿过生物膜的易位需要靶向和易位机制之间的协同作用。一个保守的膜通道，Sec61/SecY 复合物，介导真核生物内质网膜和原核生物质膜上的蛋白质易位或整合。最近的生化和结构数据的结合为通道如何允许多肽进入细胞外空间和脂双层的机制提供了新的见解。
