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两种新方法在儿童呼吸道合胞病毒感染诊断中的应用。

Usefulness of two new methods for diagnosing metapneumovirus infections in children.

机构信息

Microbiology Department, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.

出版信息

Clin Microbiol Infect. 2010 Nov;16(11):1663-8. doi: 10.1111/j.1469-0691.2010.03192.x.

Abstract

Human metapneumovirus (hMPV) is associated with acute respiratory tract infections, mainly in paediatric patients. The aim of this study was to evaluate the usefulness of two new commercial techniques available for the detection of hMPV in clinical samples from children: an enzyme immunoassay, hMPV EIA (Biotrin International Ltd), and a molecular assay, real-time RT-PCR (Pro hMPV Real Time Assay Kit; Prodesse). A total of 184 nasopharyngeal aspirate specimens from 173 children aged less than 5 years who were hospitalized with acute wheezing were analysed. Respiratory syncytial virus was detected in 27% of the samples, followed by influenza A virus (6%), parainfluenza virus (PIV)3 (2.2%), adenovirus (2%), PIV1 (1.1%), PIV2 (1.1%), and influenza B virus (0.5%). The presence of hMPV was tested in all samples, using the real-time RT-PCR and EIA. Real-time RT-PCR detected 13 hMPV-positive samples (8%), and EIA detected 17 (9.3%). When the EIA results were compared with those of real-time RT-PCR for the detection of hMPV, a good correlation was found (94%). A relatively low co-infection rate (15%) was observed in our patients. RT-PCR and EIA provide robust methods for the diagnosis of hMPV infection in children.

摘要

人偏肺病毒(hMPV)与急性呼吸道感染有关,主要发生在儿科患者中。本研究旨在评估两种新的商业技术在儿童临床样本中检测 hMPV 的有效性:酶免疫测定(Biotrin International Ltd 的 hMPV EIA)和分子测定(Pro hMPV Real Time Assay Kit;Prodesse 的实时 RT-PCR)。共分析了 173 名年龄小于 5 岁的急性喘息住院患儿的 184 份鼻咽抽吸标本。在 27%的样本中检测到呼吸道合胞病毒,其次是甲型流感病毒(6%)、副流感病毒 3(2.2%)、腺病毒(2%)、副流感病毒 1(1.1%)、副流感病毒 2(1.1%)和乙型流感病毒(0.5%)。使用实时 RT-PCR 和 EIA 检测所有样本中 hMPV 的存在。实时 RT-PCR 检测到 13 份 hMPV 阳性样本(8%),EIA 检测到 17 份(9.3%)。当比较 EIA 结果与实时 RT-PCR 检测 hMPV 时,发现相关性良好(94%)。在我们的患者中观察到相对较低的合并感染率(15%)。RT-PCR 和 EIA 为儿童 hMPV 感染的诊断提供了可靠的方法。

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