Equine Clinic, Department of Veterinary Clinical Sciences, Vetsuisse-Faculty, University of Berne, Switzerland.
Equine Vet J. 2010 Mar;42(2):142-8. doi: 10.2746/042516409X479586.
The horse owner assessed respiratory signs index (HOARSI-1-4, healthy, mildly, moderately and severely affected, respectively) is based on owner-reported clinical history and has been used for the investigation of recurrent airway obstruction (RAO) genetics utilising large sample sizes. Reliable phenotype identification is of paramount importance in genetic studies. Owner reports of respiratory signs have shown good repeatability, but the agreement of HOARSI with an in-depth examination of the lower respiratory tract has not been investigated.
To determine the correlation of HOARSI grades 3/4 with the characteristics of RAO and of HOARSI-2 with the characteristics of inflammatory airway disease. Further, to test whether there are phenotypic differences in the manifestation of lung disease between families.
Seventy-one direct offspring of 2 RAO-affected Warmblood stallions (33 from the first family, 38 from the second) were graded as HOARSI-1-4 and underwent a clinical examination of the respiratory system, arterial blood gas analysis, endoscopic mucus scoring, cytology of tracheobronchial secretion (TBS) and bronchoalveolar lavage fluid (BALF), and clinical assessment of airway reactivity to methacholine chloride.
HOARSI-3/4 animals in clinical exacerbation showed signs consistent with RAO: coughing, nasal discharge, abnormal lung sounds and breathing pattern as well as increased numbers of neutrophils in TBS and BALF, excessive mucus accumulation and airway hyper-responsiveness to methacholine. HOARSI-3/4 horses in remission only had increased amounts of tracheal mucus and TBS neutrophil percentages. Clinical phenotypes were not significantly different between the 2 families.
HOARSI reliably identifies RAO-affected horses in our population.
马主评估的呼吸系统症状指数（HOARSI-1-4，分别代表健康、轻度、中度和重度受影响）基于马主报告的临床病史，并已用于利用大样本量研究复发性气道阻塞（RAO）的遗传学。可靠的表型识别在遗传研究中至关重要。马主报告的呼吸系统症状显示出良好的可重复性，但 HOARSI 与下呼吸道的深入检查之间的一致性尚未得到研究。
确定 HOARSI 3/4 级与 RAO 的特征的相关性，以及 HOARSI-2 与炎症性气道疾病的特征的相关性。此外，检验在肺病表现方面不同家族之间是否存在表型差异。
71 匹直接后代的 2 匹 RAO 受影响的温血种马（第一个家族 33 匹，第二个家族 38 匹）被分为 HOARSI-1-4 级，并进行呼吸系统临床检查、动脉血气分析、内窥镜黏液评分、气管支气管分泌物（TBS）和支气管肺泡灌洗液（BALF）细胞学检查，以及对乙酰甲胆碱氯的气道反应性进行临床评估。
临床恶化的 HOARSI-3/4 动物表现出与 RAO 一致的症状：咳嗽、鼻腔分泌物、异常肺音和呼吸模式，以及 TBS 和 BALF 中性粒细胞数量增加、过度黏液积聚和对乙酰甲胆碱的气道高反应性。缓解期的 HOARSI-3/4 马仅存在气管黏液增加和 TBS 中性粒细胞百分比增加。两个家族之间的临床表型没有显著差异。
HOARSI 在我们的人群中可靠地识别出 RAO 受影响的马。
