Yuzuncu Yil University, Faculty of Engineering and Architecture, Chemical Engineering Department, 65080 Van, Turkey.
Bioresour Technol. 2010 Jun;101(12):4520-6. doi: 10.1016/j.biortech.2010.01.105. Epub 2010 Feb 13.
A multi-step response surface methodology was successfully applied to optimize the biosorption conditions for the maximum removal of Cu(II) ions from aqueous solutions using Trametes versicolor fungi as a biosorbent. In the first step, the most effective medium factors, which are pH, temperature and initial Cu(II) concentration, on biosorption of Cu(II), were determined through Plackett-Burman Design. Then steepest accent followed by central composite design steps were utilized to evaluate the optimum biosorption conditions for the maximum Cu(II) ions removal. Based on the statistic analysis; the optimum conditions were obtained 5.51, 20.13 degrees C and 60.98 mg/L as medium pH, medium temperature and initial Cu(II) concentration, respectively. Finally the analysis of variance (ANOVA) of central composite design showed the proposed quadratic model fitted experimental data very well.
采用多步响应面法，成功优化了利用糙皮侧耳真菌作为生物吸附剂从水溶液中最大去除 Cu(II)离子的生物吸附条件。在第一步中，通过 Plackett-Burman 设计确定了对 Cu(II)生物吸附最有效的介质因素，即 pH、温度和初始 Cu(II)浓度。然后，采用最陡爬坡和中心复合设计步骤来评估最大 Cu(II)离子去除率的最佳生物吸附条件。基于统计分析；获得的最佳条件分别为介质 pH、介质温度和初始 Cu(II)浓度为 5.51、20.13°C 和 60.98mg/L。最后，中心复合设计的方差分析（ANOVA）表明，所提出的二次模型很好地拟合了实验数据。
