Jorge Janaina Habib, Giampaolo Eunice Teresinha, Vergani Carlos Eduardo, Pavarina Ana Cláudia, Machado Ana Lúcia, Carlos Iracilda Zeppone
Department of Dentistry, POnta Grossa State University, Parana, Brazil.
Quintessence Int. 2009 Nov-Dec;40(10):e93-100.
To evaluate the effect of microwave postpolymerization heat treatment and water storage time on the cytotoxicity of denture base and reline acrylic resins.
Sample disks of 6 acrylic resins were fabricated under aseptic conditions (Kooliner, GC; Tokuyama Rebase II, Tokuyama Dental; New Truliner, Bosworth; Acron MC, GC; Lucitone 550, Dentsply; QC 20, Dentsply). The denture base acrylic resin samples were stored in water for 24 and 48 hours at 37 degrees C. The fabricated samples were further subdivided into 2 groups: (1) samples without heat treatment and (2) samples treated in a microwave. The 3H-thymidine incorporation test was used to determine the cytotoxicity of the materials. The data were statistically analyzed using the incomplete 3-way ANOVA and Tukey HSD tests (P < .05).
Microwave postpolymerization heat treatment improved the biocompatibility of Tokuyama Rebase II. Tokuyama Rebase II without heat treatment and Acron MC in both experimental groups were graded as slightly cytotoxic in the group without water storage. The other resins were graded as noncytotoxic. After 24 hours of immersion in water, all materials were graded as noncytotoxic. After water storage for 48 hours, Acron MC without heat treatment and QC 20 in both experimental groups were graded as slightly cytotoxic. Lucitone 550 was graded as noncytotoxic in all experimental groups.
Microwave irradiation may be considered an alternative to reduce the cytotoxicity of Tokuyama Rebase II. Dental practitioners should choose Lucitone 550 processed with terminal boiling stage.
评估微波聚合后热处理及水储存时间对义齿基托和重衬丙烯酸树脂细胞毒性的影响。
在无菌条件下制作6种丙烯酸树脂的样本盘（Kooliner，GC公司；Tokuyama Rebase II，德山齿科；New Truliner，博斯沃思公司；Acron MC，GC公司；Lucitone 550，登士柏公司；QC 20，登士柏公司）。义齿基托丙烯酸树脂样本在37℃水中储存24小时和48小时。制作的样本进一步分为2组：（1）未热处理样本和（2）微波处理样本。采用³H-胸腺嘧啶核苷掺入试验测定材料的细胞毒性。数据采用不完全三因素方差分析和Tukey HSD检验进行统计学分析（P < 0.05）。
微波聚合后热处理改善了Tokuyama Rebase II的生物相容性。未热处理的Tokuyama Rebase II和两个实验组中的Acron MC在未储存水的组中被评为轻度细胞毒性。其他树脂被评为无细胞毒性。在水中浸泡24小时后，所有材料均被评为无细胞毒性。储存水48小时后，未热处理的Acron MC和两个实验组中的QC 20被评为轻度细胞毒性。Lucitone 550在所有实验组中均被评为无细胞毒性。
微波辐照可被视为降低Tokuyama Rebase II细胞毒性的一种替代方法。牙科医生应选择经过终沸点处理的Lucitone 550。
