用回收的聚甲基丙烯酸甲酯基义齿基托树脂改性的热固化义齿基托树脂的细胞毒性评估

Evaluation of the Cytotoxicity of Heat Cure Denture Base Resin Modified with Recycled PMMA-Based Denture Base Resin.

作者信息

Krishnamoorthi Divya, Karthigeyan Suma, Ali Seyed Asharaf, Rajajayam Sasikala, Gajendran Ramya, Rajendran Mithra

机构信息

Department of Prosthodontics and Crown and Bridge, Vinayaka Mission's Sankarachariyar Dental College, A Constituent College of Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanoor, Tamil Nadu, India.

Department of Prosthodontics, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.

出版信息

J Pharm Bioallied Sci. 2022 Jul;14(Suppl 1):S719-S725. doi: 10.4103/jpbs.jpbs_890_21. Epub 2022 Jul 13.

DOI:10.4103/jpbs.jpbs_890_21

PMID:36110705

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9469223/

Abstract

AIM

The aim of the present study was to evaluate the cytotoxicity of heat-cure denture base resin (PMMA) modified with recycled denture base resin at 10%, 20%, 30%, 40%, and 50% (w/w) concentration.

MATERIALS AND METHODS

A total of 30 disk-shaped specimens were prepared and divided into six groups ( = 5). The Control group (R) consisted of unmodified processed denture base resin, the experimental group consisted of denture base resin processed with substitution of 10% 20%, 30%, 40%, and 50% (w/w) of recycled denture base resin (R, R R R, and R). Eluates were prepared using five sterile specimens of each group. The mouse fibroblast cell line (L929) was seeded in a 96-well cell plate system at a concentration of 1 × 10 cells/well in the DMEM medium with 1× antibiotic and antimycotic solution and 10% fetal bovine serum at 37°C with 5% CO and incubated in a CO incubator for 48 h. MTT assay was applied and the absorbance was measured at 570 nm using a microplate reader to assess the cytotoxicity. One-way analysis of variance (ANOVA) along with post hoc Scheffe test was used to statistically compare the mean optical density (OD) values and cell survival/viability % amongst the groups.

RESULTS

No statistically significant difference was observed in the mean and standard deviation of the optical density and cell viability % of the test groups that were compared.

CONCLUSION

Modification of denture base resin using recycled PMMA does not have a cytotoxic effect on the mouse fibroblast cell line L929.

摘要

目的

本研究旨在评估以10%、20%、30%、40%和50%（重量/重量）浓度的回收义齿基托树脂改性的热固化义齿基托树脂（聚甲基丙烯酸甲酯）的细胞毒性。

材料与方法

共制备30个圆盘形标本，分为六组（每组n = 5）。对照组（R）由未改性的加工义齿基托树脂组成，实验组由分别用10%、20%、30%、40%和50%（重量/重量）的回收义齿基托树脂（R1、R2、R3、R4和R5）替代加工的义齿基托树脂组成。使用每组的五个无菌标本制备洗脱液。将小鼠成纤维细胞系（L929）以1×10⁴个细胞/孔的浓度接种于96孔细胞培养板系统中，置于含有1×抗生素和抗真菌溶液以及10%胎牛血清的DMEM培养基中，在37°C、5% CO₂条件下于CO₂培养箱中孵育48小时。应用MTT法，使用酶标仪在570 nm处测量吸光度以评估细胞毒性。采用单因素方差分析（ANOVA）以及事后Scheffe检验对各组间的平均光密度（OD）值和细胞存活/活力百分比进行统计学比较。