LI-COR Biosciences, Inc., 4647 Superior Street, Lincoln, Nebraska 68504, USA.
Nano Lett. 2010 Mar 10;10(3):788-92. doi: 10.1021/nl9039718.
Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.
高度标记的 DNA 纳米球与磷酸化核苷酸三磷酸(dNTP)连接,作为 DNA 聚合酶的 dNTP 来源。这些颗粒是通过自互补的圆形模板的链置换聚合制备的。原子力显微镜成像显示,这些功能化的颗粒呈现出直径在 50 至 150nm 之间的凝聚模糊球。当使用 TIR 荧光显微镜成像时,它们发出明亮的荧光信号,在 2ms 的曝光时间内,信噪比为 25。