College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801 Shanxi, PR China.
Toxicol Mech Methods. 2010 Feb;20(2):75-81. doi: 10.3109/15376510903559968.
Acrylamide (ACR) and high contents of fat could be found co-existent in many foods processed by high temperature, such as deep-frying and roasting. This study investigated the effect of enhanced fat consumption on deficits of spermatogenesis induced by ACR, and explored potential mechanisms of oxidative damage involved in this pathology in mice. Results show that enhanced feeding of corn oil and pork fat on mice potentiated the decreases of spermatogonia along with mature sperms after treatment of ACR, and that spermatozoa quality is significantly reduced as a result of enhanced feeding of corn oil and pork fat on mice treated with ACR. Moreover, enhanced consumption of corn oil and pork fat potentiated the up-regulation of malondialdehyde (MDA) level in epididymal sperm and cauda epididymides, also up-regulated level of Protein carbonyls (PCOs) in cauda epididymides, of mice after treatment of ACR. Last, enhanced consumption of corn oil and pork fat potentiated the reduced activity of superoxide dismutases (SOD) in epididymal sperm, corpus, and cauda epididymides, also reduced activity of glutathione peroxidase (GPx) in cauda epididymides, of mice treated with ACR. These data suggest that enhanced feeding of corn oil and pork fat on mice potentiates ACR-induced oxidative stress in the epididymis and epididymal sperm and a subsequent effect on spermatogenesis.
丙烯酰胺（ACR）和高脂肪含量可以在许多经过高温处理的食物中同时发现，例如油炸和烤制。本研究探讨了增强脂肪摄入对丙烯酰胺引起的精子发生缺陷的影响，并探索了这种病理学中涉及氧化损伤的潜在机制。结果表明，在 ACR 处理后，给小鼠增强玉米油和猪油的喂养会加剧精原细胞和成熟精子的减少，并且由于 ACR 处理后给小鼠增强玉米油和猪油的喂养，精子质量显著降低。此外，增强玉米油和猪油的消耗会加剧 ACR 处理后附睾精子和尾部附睾中丙二醛（MDA）水平的上调，也会增加尾部附睾中蛋白羰基（PCOs）的水平。最后，增强玉米油和猪油的消耗会加剧 ACR 处理后尾部附睾中超氧化物歧化酶（SOD）的活性降低， corpus 和尾部附睾，也降低了尾部附睾中谷胱甘肽过氧化物酶（GPx）的活性。这些数据表明，在 ACR 处理后，给小鼠增强玉米油和猪油的喂养会加剧附睾和附睾精子中的氧化应激，进而对精子发生产生影响。
