Chitra K C, Rao K Ramachandra, Mathur P P
School of Life Sciences, Pondicherry University, Pondicherry 605 014, India.
Asian J Androl. 2003 Sep;5(3):203-8.
To study the effect of bisphenol A on the epididymis and epididymal sperm of rats and the possible amelioration action of co-administration with vitamin C.
Male Wistar rats were orally administered bisphenol A (0.2 microg x kg (-1) x day(-1), 2 microg x kg(-1) x day(-1) and 20 microg x kg(-1) x day(-1)) and 0.2 microg, 2 microg and 20 microg bisphenol A + 40 mg vitamin C x kg(-1) x day(-1) for 60 days. On day 61, rats were killed with anesthetic ether and sperm collected from epididymis were used or assessment of sperm count, motility and viability and biochemical studies. A 1 % homogenate of epididymis was prepared and used for biochemical estimations. Caput, corpus and cauda epididymis were fixed in Bouin's fixative for histological studies.
Administration of bisphenol A caused a reduction in the epididymal sperm motility and count and the sperm viability remained unchanged. The activities of superoxide dismutase and glutathione peroxidase decreased, while the levels of lipid peroxidation increased in epididymal sperm and epididymis at all doses. Co-administration with vitamin C reversed the effect of bisphenol A-induced oxidative stress in epididymal sperm and epididymis. A complete degeneration of epididymal epithelium in caput, corpus and cauda regions with reduction in the number of sperms were observed at all doses of bisphenol A-treated rats.
Bisphenol A induced oxidative stress in epididymis and caused degeneration of the epididymal epithelium of rats. Co-administration with vitamin C had a protective effect against the bisphenol A-induced toxicity in epididymal sperm and epididymis.
研究双酚A对大鼠附睾及附睾精子的影响，以及与维生素C共同给药可能的改善作用。
将雄性Wistar大鼠口服给予双酚A（0.2微克×千克⁻¹×天⁻¹、2微克×千克⁻¹×天⁻¹和20微克×千克⁻¹×天⁻¹）以及0.2微克、2微克和20微克双酚A + 40毫克维生素C×千克⁻¹×天⁻¹，持续60天。在第61天，用麻醉乙醚处死大鼠，收集附睾中的精子用于评估精子数量、活力和存活率以及进行生化研究。制备1%的附睾匀浆用于生化测定。将附睾头、体和尾固定在Bouin固定液中用于组织学研究。
给予双酚A导致附睾精子活力和数量减少，精子存活率保持不变。所有剂量下，附睾精子和附睾中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性降低，而脂质过氧化水平升高。与维生素C共同给药可逆转双酚A诱导的附睾精子和附睾氧化应激效应。在所有剂量的双酚A处理大鼠中，均观察到附睾头、体和尾区域的附睾上皮完全退化，精子数量减少。
双酚A在大鼠附睾中诱导氧化应激并导致附睾上皮退化。与维生素C共同给药对双酚A诱导的附睾精子和附睾毒性具有保护作用。
