Rojo M, Hovius R, Demel R, Wallimann T, Eppenberger H M, Nicolay K
Institute for Cell Biology, Eidgenössische Technische Hochschule, Zürich, Switzerland.
FEBS Lett. 1991 Apr 9;281(1-2):123-9. doi: 10.1016/0014-5793(91)80374-c.
The interaction of mitochondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi-CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligomeric form of Mi-CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi-CK was able to interact equally well with both inner and outer mitochondrial membranes.
已对线粒体肌酸激酶（Mi-CK；EC 2.7.3.2）与磷脂单层以及在空气/水界面铺展的线粒体膜之间的相互作用进行了研究。结果表明，Mi-CK可渗透到这些单层中，这可通过加入Mi-CK后表面压力的增加得以证明。表面压力的增加取决于：（1）亚相中Mi-CK的量；（2）Mi-CK的寡聚形式；（3）初始表面压力；以及（4）单层的磷脂组成。在该实验系统中，Mi-CK与线粒体内膜和外膜的相互作用同样良好。
