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Rapid aggregation and tight junction formation in single cell suspensions of tumor cells after very low dose trypsin treatment.

作者信息

Kemmner W, Zaar K

机构信息

Institut für Biochemie II, Universität Heidelberg, Germany.

出版信息

FEBS Lett. 1991 Apr 9;281(1-2):43-6. doi: 10.1016/0014-5793(91)80354-6.

DOI:10.1016/0014-5793(91)80354-6
PMID:2015907
Abstract

The possible participation of tight junction formation in intercellular adhesion of tumor cells was studied in single cell suspensions of HT29 adenocarcinoma cells. Very low dose trypsin treatment (0.15 micrograms/ml, 30 min, 37 degrees C) induced rapid intercellular adhesion of suspended HT29 colon carcinoma cells. Intercellular adhesion was independent of the presence of divalent cations. Electron microscopy of freeze-fractured membrane fragments of trypsin-treated HT29 cells revealed a dramatic increase of typical tight junction structures during aggregation.

摘要

相似文献

1
Rapid aggregation and tight junction formation in single cell suspensions of tumor cells after very low dose trypsin treatment.
FEBS Lett. 1991 Apr 9;281(1-2):43-6. doi: 10.1016/0014-5793(91)80354-6.
2
Degradation of tight junctions in HT29, a human colon adenocarcinoma cell line.
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Formation of tight junctions in epithelial cells. I. Induction by proteases in a human colon carcinoma cell line.
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