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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Itoh N, Mori K, Toga K, Ikegami S

Faculty of Applied Biological Sciences, Hiroshima University, Japan.

Int J Biochem. 1991;23(4):491-7.

Murine dihydrofolate reductase was produced efficiently in a Xenopus laevis oocyte that received microinjection of the dihydrofolate reductase mRNA. 2. Murine dihydrofolate reductase was produced under the direction of the plasmid pAdD26SVpA, which contains adenovirus major late promoter and the simian virus 40 T antigen gene for transcription initiation and murine dehydrofolate reductase complementary DNA fragment. 3. Injection of the plasmid pDHFR26, which contains murine dihydrofolate reductase complementary DNA and normal transcription initiation site segment in the 5' untranslational region, failed to produce proper translational products due to improper transcription.

小鼠二氢叶酸还原酶在通过显微注射二氢叶酸还原酶mRNA的非洲爪蟾卵母细胞中高效产生。2. 小鼠二氢叶酸还原酶在质粒pAdD26SVpA的指导下产生，该质粒含有腺病毒主要晚期启动子和用于转录起始的猴病毒40 T抗原基因以及小鼠二氢叶酸还原酶互补DNA片段。3. 注射质粒pDHFR26（其在5'非翻译区含有小鼠二氢叶酸还原酶互补DNA和正常转录起始位点片段）未能产生合适的翻译产物，因为转录不当。

Itoh N, Mori K, Toga K, Ikegami S

Faculty of Applied Biological Sciences, Hiroshima University, Japan.

Int J Biochem. 1991;23(4):491-7.

Murine dihydrofolate reductase was produced efficiently in a Xenopus laevis oocyte that received microinjection of the dihydrofolate reductase mRNA. 2. Murine dihydrofolate reductase was produced under the direction of the plasmid pAdD26SVpA, which contains adenovirus major late promoter and the simian virus 40 T antigen gene for transcription initiation and murine dehydrofolate reductase complementary DNA fragment. 3. Injection of the plasmid pDHFR26, which contains murine dihydrofolate reductase complementary DNA and normal transcription initiation site segment in the 5' untranslational region, failed to produce proper translational products due to improper transcription.

小鼠二氢叶酸还原酶在通过显微注射二氢叶酸还原酶mRNA的非洲爪蟾卵母细胞中高效产生。2. 小鼠二氢叶酸还原酶在质粒pAdD26SVpA的指导下产生，该质粒含有腺病毒主要晚期启动子和用于转录起始的猴病毒40 T抗原基因以及小鼠二氢叶酸还原酶互补DNA片段。3. 注射质粒pDHFR26（其在5'非翻译区含有小鼠二氢叶酸还原酶互补DNA和正常转录起始位点片段）未能产生合适的翻译产物，因为转录不当。