采用鱼血清的中国仓鼠卵巢细胞无胎牛血清悬浮培养。
Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum.
机构信息
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo 060-8628, Japan.
出版信息
J Biosci Bioeng. 2010 Mar;109(3):307-9. doi: 10.1016/j.jbiosc.2009.08.481. Epub 2009 Sep 16.
PMID:20159583
Abstract
The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15(500) (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density.
摘要
研究了热处理和鱼血清（FS）浓度对重组中国仓鼠卵巢（CHO）1-15（500）（ATCC CRL-9606）细胞悬浮培养中细胞生长的影响。FS 浓度从 1%增加到 4%显著增加了细胞密度。另一方面，FS 的热处理对细胞密度几乎没有影响。
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