[Construction and expression of the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217].

OBJECTIVE

To construct the eukaryotic green fluorescent protein expression vector pEGFP-N1-ZNF217 and express the vector in eukaryotic cells.

METHODS

ZNF217 gene fragment was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), and after analysis of the product by electrophoresis and sequencing, the fragment was inserted into pEGFP-N1 fluorescent expression vector. The constructed expression vector was then transfected into eukaryotic cells for its expression.

RESULTS AND CONCLUSION

Restriction endonuclease digestion and sequence analysis confirmed correct construction of the recombinant vector pEGFP-N1 and the expression vector pEGFP-N1-ZNF217, which can be stably expressed in eukaryotic cells.