Department of Biological Sciences, Center for Molecular Neuroscience, Institutes of Chemical Biology and Global Health, Vanderbilt University, Nashville, TN 37235, USA.
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4418-23. doi: 10.1073/pnas.0913392107. Epub 2010 Feb 16.
A systematic functional analysis across much of the conventional Anopheles gambiae odorant receptor (AgOR) repertoire was carried out in Xenopus oocytes using two-electrode, voltage-clamp electrophysiology. The resulting data indicate that each AgOR manifests a distinct odor-response profile and tuning breadth. The large diversity of tuning responses ranges from AgORs that are responsive to a single or small number of odorants (specialists) to more broadly tuned receptors (generalists). Several AgORs were identified that respond robustly to a range of human volatiles that may play a critical role in anopheline host selection. AgOR responses were analyzed further by constructing a multidimensional odor space representing the relationships between odorants and AgOR responses. Within this space, the distance between odorants is related to both chemical class and concentration and may correlate with olfactory discrimination. This study provides a comprehensive overview of olfactory coding mechanisms of An. gambiae that ultimately may aid in fostering the design and development of olfactory-based strategies for reducing the transmission of malaria and other mosquito-borne diseases.
采用双电极电压钳电生理学技术，在非洲疟蚊（Anopheles gambiae）气味受体（AgOR）的大部分传统受体中进行了系统功能分析。结果表明，每个 AgOR 都表现出独特的气味反应特征和调谐宽度。调谐反应的多样性范围从只对一种或少数气味有反应的 AgOR（专家）到更广泛调谐的受体（通才）。鉴定出一些 AgOR 对一系列人类挥发物有强烈反应，这些挥发物可能在按蚊宿主选择中起着关键作用。通过构建多维气味空间来进一步分析 AgOR 反应，该空间代表了气味剂与 AgOR 反应之间的关系。在这个空间中，气味剂之间的距离与化学类别和浓度有关，可能与嗅觉辨别有关。这项研究全面概述了非洲疟蚊的嗅觉编码机制，最终可能有助于设计和开发基于嗅觉的策略，以减少疟疾和其他蚊媒疾病的传播。
