Cooper D L, Baptist E W, Enghild J J, Isola N R, Klintworth G K
Department of Pathology, Duke University, Durham, NC 27710.
Gene. 1991 Feb 15;98(2):201-7. doi: 10.1016/0378-1119(91)90174-a.
Amino acid (aa) sequence data from Staphylococcus areas V8 protease-digested bovine corneal 54-kDa protein (BCP54) fragments were utilized to derive mixed oligodeoxyribonucleotide (oligo) primers complementary to the reverse translation products of these sequences. These degenerate oligo primers were used to prime the amplification of BCP54 sequence from bovine corneal epithelial cell cDNA. The cDNA probe generated by this mixed oligo-primed amplification of cDNA was cloned and dideoxy-sequenced. A search of the GenBank database (version 63.0) revealed extensive sequence similarity to the cDNA encoding tumor-associated rat liver (class 3) aldehyde dehydrogenase (RATALD). Nucleotide (nt) and aa sequence alignment of the BCP54 translation product reveals it is 78% and 84% homologous with RATALD at the nt and aa levels, respectively. Conservation of aa sequence elements common to the aldehyde dehydrogenase family thought to be of structural/functional significance is further substantiated by this analysis. Included in the discussion is the likelihood that gene sharing (genes encoding metabolic enzymes and other stable proteins) may extend to the cornea.
利用来自金黄色葡萄球菌V8蛋白酶消化的牛角膜54-kDa蛋白（BCP54）片段的氨基酸（aa）序列数据，推导与这些序列的反向翻译产物互补的混合寡聚脱氧核糖核苷酸（oligo）引物。这些简并寡核苷酸引物用于从牛角膜上皮细胞cDNA中扩增BCP54序列。通过这种混合寡核苷酸引发的cDNA扩增产生的cDNA探针被克隆并进行双脱氧测序。对GenBank数据库（版本63.0）的搜索显示，其与编码肿瘤相关大鼠肝脏（3类）醛脱氢酶（RATALD）的cDNA具有广泛的序列相似性。BCP54翻译产物的核苷酸（nt）和氨基酸序列比对显示，其在nt和aa水平上分别与RATALD具有78%和84%的同源性。该分析进一步证实了醛脱氢酶家族共有的氨基酸序列元件的保守性，这些元件被认为具有结构/功能意义。讨论内容还包括基因共享（编码代谢酶和其他稳定蛋白的基因）可能延伸至角膜的可能性。
