Tseng Ta-Feng, Yang Yang-Li, Chuang Min-Chieh, Lou Shyh-Liang, Galik Michal, Flechsig Gerd-Uwe, Wang Joseph
Department of Nanoengineering, University of California San Diego, La Jolla, CA, 92093, USA.
Electrochem commun. 2009 Sep 1;11(9):1819-1822. doi: 10.1016/j.elecom.2009.07.030.
We illustrate how the use of heated electrodes enhances the performance of glucose biosensors based on amperometric detection of the glucose-oxidase generated hydrogen peroxide. Nafion is shown to be an excellent matrix to protect glucose oxidase from thermal inactivation during the heating pulses. The influence of the electrode temperature upon the amperometric response is examined. Temperature pulse amperometry (TPA) has been used to obtain convenient peak-shaped analytical signals. Surprisingly, up to 67.5 °C, the activity of Nafion-entrapped glucose oxidase is greatly enhanced (24 -fold) by accelerated kinetics rather than decreased by thermal inactivation. Amperometric signals even at elevated temperatures are stable upon prolonged operation involving repetitive measurements. The linear calibration range is significantly extended.
我们展示了使用加热电极如何基于对葡萄糖氧化酶产生的过氧化氢进行安培检测来提高葡萄糖生物传感器的性能。结果表明，Nafion是一种出色的基质，可在加热脉冲期间保护葡萄糖氧化酶免受热失活。研究了电极温度对安培响应的影响。温度脉冲安培法（TPA）已被用于获得方便的峰形分析信号。令人惊讶的是，在高达67.5°C的温度下，Nafion包裹的葡萄糖氧化酶的活性通过加速动力学大大增强（24倍），而不是受热失活而降低。即使在高温下，经过长时间涉及重复测量的操作后，安培信号仍然稳定。线性校准范围也显著扩展。
