Bou Chakra Elie, Hannes Benjamin, Vieillard Julien, Mansfield Colin D, Mazurczyk Radoslav, Bouchard Aude, Potempa Jan, Krawczyk Stanislas, Cabrera Michel
Institut des Nanotechnologies de Lyon, INL UMR CNRS ECL-INSA-UCBL 5270, Ecole Centrale de Lyon, 36 avenue Guy de Collongue, F69134 Ecully, France.
Sens Actuators B Chem. 2009 Jun 18;140(1):278-286. doi: 10.1016/j.snb.2009.03.030.
A novel approach to integrating biochip and microfluidic devices is reported in which microcontact printing is a key fabrication technique. The process is performed using an automated microcontact printer that has been developed as an application-specific tool. As proof-of-concept the instrument is used to consecutively and selectively graft patterns of antibodies at the bottom of a glass channel for use in microfluidic immunoassays. Importantly, feature collapse due to over compression of the PDMS stamp is avoided by fine control of the stamp's compression during contact. The precise alignment of biomolecules at the intersection of microfluidic channel and integrated optical waveguides has been achieved, with antigen detection performed via fluorescence excitation. Thus, it has been demonstrated that this technology permits sequential microcontact printing of isolated features consisting of functional biomolecules at any position along a microfluidic channel and also that it is possible to precisely align these features with existing components.
报道了一种集成生物芯片和微流控设备的新方法，其中微接触印刷是关键的制造技术。该过程使用作为特定应用工具开发的自动微接触打印机来执行。作为概念验证，该仪器用于在玻璃通道底部连续且选择性地接枝抗体图案，以用于微流控免疫分析。重要的是，通过在接触过程中精细控制印章的压缩，避免了由于PDMS印章过度压缩导致的特征塌陷。已实现生物分子在微流控通道与集成光波导交叉处的精确对准，并通过荧光激发进行抗原检测。因此，已经证明该技术允许在微流控通道沿线的任何位置对由功能性生物分子组成的孤立特征进行顺序微接触印刷，并且还能够将这些特征与现有组件精确对准。
