将甘蔗线条病毒鉴定为一种独特的双生病毒。

Characterization of the sugarcane streak agent as a distinct geminivirus.

作者信息

Hughes F L, Rybicki E P, Kirby R, von Wechmar M B

机构信息

Department of Microbiology, University of Cape Town: Republic of South Africa.

出版信息

Intervirology. 1991;32(1):19-27. doi: 10.1159/000150181.

DOI:10.1159/000150181

PMID:2016148

Abstract

The relationship between maize streak virus and the geminivirus causing streak in sugarcane was investigated. The DNA of sugarcane streak virus does not cross-hybridize detectably with that of maize streak virus and vice versa. Restriction mapping of native replicative form viral DNA (genome size 2.7 kb) and of cloned viral DNA, combined with limited sequencing and estimated DNA sequence divergence, showed that sugarcane streak virus is as unrelated to maize streak virus and digitaria streak virus as these are different from each other. The virus is only distantly related to wheat dwarf virus and chloris striate mosaic virus. Based on these results, we propose that the agent causing sugarcane streak is a distinct geminivirus.

摘要

对玉米条纹病毒与导致甘蔗条纹病的双生病毒之间的关系进行了研究。甘蔗条纹病毒的DNA与玉米条纹病毒的DNA之间未检测到明显的交叉杂交，反之亦然。对天然复制型病毒DNA（基因组大小为2.7 kb）和克隆的病毒DNA进行限制性图谱分析，并结合有限的测序和估计的DNA序列差异，结果表明甘蔗条纹病毒与玉米条纹病毒和马唐草条纹病毒的关系并不比它们彼此之间的关系更近。该病毒与小麦矮缩病毒和虎尾草条纹花叶病毒仅存在远缘关系。基于这些结果，我们提出导致甘蔗条纹病的病原体是一种独特的双生病毒。