用于人类胚胎干细胞的可扩展培养系统的开发。
Development of Scalable Culture Systems for Human Embryonic Stem Cells.
作者信息
Azarin Samira M, Palecek Sean P
机构信息
Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Drive, Madison, WI 53706.
出版信息
Biochem Eng J. 2010 Feb 15;48(3):378. doi: 10.1016/j.bej.2009.10.020.
Abstract
The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in large-scale cultures of other mammalian cells will facilitate expansion of undifferentiated human embryonic stem cells (hESCs), but challenges specific to hESCs will also have to be addressed, including development of defined, humanized culture media and substrates, monitoring spontaneous differentiation and heterogeneity in the cultures, and maintaining karyotypic integrity in the cells. This review will describe our current understanding of environmental factors that regulate hESC self-renewal and efforts to provide these cues in various scalable bioreactor culture systems.
摘要
在治疗应用中使用人类多能干细胞，包括胚胎干细胞和诱导多能干细胞，将需要开发适用于未分化细胞的强大、可扩展的培养技术。其他哺乳动物细胞大规模培养方面取得的进展将有助于未分化的人类胚胎干细胞（hESCs）的扩增，但hESCs特有的挑战也必须加以解决，包括开发明确的、人源化的培养基和底物，监测培养物中的自发分化和异质性，以及维持细胞中的核型完整性。本综述将描述我们目前对调节hESC自我更新的环境因素的理解，以及在各种可扩展生物反应器培养系统中提供这些信号的努力。
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