使用第二代BD钙检测试剂盒对G蛋白偶联受体进行活细胞分析的改进。

Li Xiao, Llorente Isabel, Brasch Mike

Bioimaging Systems, BD Biosciences, Rockville, Maryland, USA.

Curr Chem Genomics. 2008 Oct 10;2:10-5. doi: 10.2174/1875397300802010010.

BD Calcium Assay Kits are designed for cell-based calcium mobilization high-throughput screening assays. The kits use a proprietary formulation including a non-fluorescent calcium indicator that becomes activated inside the cell and shows increased fluorescence upon calcium binding. The formulation includes a signal-enhancing reagent to maximize the signal over background in a homogeneous, no-wash assay format, based on a technology developed at BD. We have compared the next generation BD calcium assay kit product family to previous versions of the formulation, and to other commercially available homogeneous calcium assay kits. The improvements have enabled better performance on the cell lines and receptors that we have tested in all plate formats including 1536.

BD钙检测试剂盒专为基于细胞的钙动员高通量筛选检测而设计。这些试剂盒采用了一种专利配方，其中包括一种非荧光钙指示剂，该指示剂在细胞内被激活，并在与钙结合时显示出荧光增强。该配方包括一种信号增强试剂，基于BD开发的技术，以在均相、无需洗涤的检测形式中使信号相对于背景最大化。我们已将下一代BD钙检测试剂盒产品系列与该配方的先前版本以及其他市售均相钙检测试剂盒进行了比较。这些改进使得在我们测试的所有板型（包括1536孔板）中的细胞系和受体上都有更好的性能表现。