Combs N R, Kornegay E T, Lindemann M D, Notter D R, Welker F H
Dept. of Anim. Sci., Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Anim Sci. 1991 Feb;69(2):664-72. doi: 10.2527/1991.692664x.
Tibia biopsies were taken from 75 live pigs at 4-wk intervals and from 251 slaughtered pigs to evaluate bone biopsy as a procedure for determining Ca/P status in pigs fed 70, 85, 100, 115 and 130% of the NRC (1979) estimated dietary Ca and P percentage requirements from weaning to market. Least squares means and SE of live and slaughter biopsy wet weight, ash weight and dry, fat-free ash percentage (DFF%) were compared at each time in each trial and found not to differ. Diet and time effects on ash weight, ash percentage of wet weight and DFF% of the biopsy core also did not differ greatly between slaughter and live biopsies and generally responded linearly and quadratically (P less than .01) to increasing Ca/P level and time. Biopsy measures were correlated (P less than .05) with third and fourth metacarpal and metatarsal length, bending and shear stress and DFF%. Means for slaughter biopsy DFF% did not differ greatly from the average of third and fourth metacarpal and metatarsal DFF% from slaughter pigs. Means for live and slaughter biopsy DFF% were lower than those for whole bones for the 70 and 85% NRC estimated Ca/P levels, but not for the 100, 115 and 130% NRC levels. Bone biopsy offers potential as a reliable noninvasive procedure for monitoring Ca/P status of swine from weaning to market, but it needs further study for use in Ca/P research in swine.
每隔4周从75头活猪身上采集胫骨活检样本，并从251头屠宰猪身上采集样本，以评估骨活检作为一种确定从断奶到上市阶段采食美国国家研究委员会（1979）估计的日粮钙和磷百分比需求量的70%、85%、100%、115%和130%的猪的钙/磷状态的方法。在每个试验的每次时间点，对活猪和屠宰猪活检样本的湿重、灰分重量以及干的、无脂灰分百分比（DFF%）的最小二乘均值和标准误进行比较，发现并无差异。屠宰和活猪活检之间，日粮和时间对活检核心的灰分重量、湿重灰分百分比和DFF%的影响也没有很大差异，并且通常随着钙/磷水平和时间的增加呈线性和二次响应（P小于0.01）。活检测量值与第三和第四掌骨及跖骨长度、弯曲和剪切应力以及DFF%相关（P小于0.05）。屠宰猪活检DFF%的均值与屠宰猪第三和第四掌骨及跖骨DFF%的平均值差异不大。对于美国国家研究委员会估计的钙/磷水平为70%和85%的情况，活猪和屠宰猪活检DFF%的均值低于全骨的均值，但对于100%、115%和130%的美国国家研究委员会水平则不然。骨活检作为一种从断奶到上市监测猪钙/磷状态的可靠非侵入性方法具有潜力，但在猪的钙/磷研究中的应用还需要进一步研究。
