Manoharan Shanmugam, Panjamurthy Kuppusamy, Pugalendi Pachaiappan, Balakrishnan Subramanian, Rajalingam Kasinathan, Vellaichamy Lakshmanan, Alias Linsa Mary
Department of Biochemistry & Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):94-102. doi: 10.4314/ajtcam.v6i1.57079.
The aim of the present study was to investigate the protective effect of Withaferin-A on red blood cell integrity during 7,12-dimethylbenz[a]anthracene (DMBA) induced oral carcinogenesis. The protective effect of Withaferin-A was assessed by measuring the status of glycoconjugates, membrane bound enzyme activity and red blood cell osmotic fragility. Oral squamous cell carcinoma was induced in the buccal pouch of Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin thrice a week for 14 weeks. The levels of glycoconjugates, membrane bound enzyme activity, osmotic fragility and thiobarbituric acid reactive substances (TBARS) were analyzed by using specific colorimetric methods. We observed 100% tumor formation in DMBA painted hamsters. Increase in plasma glycoconjugates at the expense of red blood cell membrane glycoconjugates levels were observed in DMBA painted hamsters as compared to control hamsters. Erythrocytes from DMBA painted hamsters were more fragile than those from control hamsters. The activity of membrane bound enzyme (Na(+) K(+) ATPase) decreased whereas TBARS level was increased in DMBA painted hamsters as compared to control hamsters. Oral administration of Withaferin-A at a dose of 20 mg kg(-1) bw significantly prevented the tumor formation as well as normalized the biochemical variables in DMBA painted hamsters. Our results thus demonstrate the protective effect of Withaferin-A on red blood cell integrity during DMBA induced oral carcinogenesis.
本研究的目的是探讨 Withaferin-A 在 7,12-二甲基苯并[a]蒽（DMBA）诱导的口腔癌发生过程中对红细胞完整性的保护作用。通过测量糖缀合物状态、膜结合酶活性和红细胞渗透脆性来评估 Withaferin-A 的保护作用。每周三次用 0.5% DMBA 的液体石蜡溶液涂抹叙利亚金仓鼠的颊囊，持续 14 周，诱导口腔鳞状细胞癌。使用特定的比色法分析糖缀合物水平、膜结合酶活性、渗透脆性和硫代巴比妥酸反应性物质（TBARS）。我们观察到涂抹 DMBA 的仓鼠肿瘤形成率为 100%。与对照仓鼠相比，涂抹 DMBA 的仓鼠血浆糖缀合物增加，而红细胞膜糖缀合物水平降低。涂抹 DMBA 的仓鼠的红细胞比对照仓鼠的红细胞更脆弱。与对照仓鼠相比，涂抹 DMBA 的仓鼠膜结合酶（Na(+) K(+) ATPase）活性降低，而 TBARS 水平升高。以 20 mg kg(-1) bw 的剂量口服 Withaferin-A 可显著预防肿瘤形成，并使涂抹 DMBA 的仓鼠的生化变量恢复正常。因此，我们的结果证明了 Withaferin-A 在 DMBA 诱导的口腔癌发生过程中对红细胞完整性的保护作用。
