Panjamurthy Kuppusamy, Manoharan Shanmugam, Balakrishnan Subramanian, Suresh Kathiresan, Nirmal Madhavan R, Senthil Namasivayam, Alias Linsa Marry
Department of Biochemistry & Biotechnology, Faculty of Science, Raja Muthaiah Dental College and Hospital, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):1-8. doi: 10.4314/ajtcam.v6i1.57067.
Our aim was to investigate the effect of Withaferin-A on bone marrow micronucleus frequency and buccal mucosa detoxication agents during 7, 12-dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral squamous cell carcinoma was developed in hamsters' buccal pouches by painting 0.5% DMBA in liquid paraffin, three times per week for 14 weeks. We observed 100% tumor formation in DMBA painted hamsters. Elevated frequency of bone marrow micronucleated polychromatic erythrocytes (MnPCEs) and decrease in buccal mucosa phase II detoxication agents were noticed in tumor bearing hamsters. Oral administration of Withaferin-A significantly reduced the micronucleus frequency and brought back the status of phase II detoxication agents in DMBA painted hamsters. Our study thus demonstrated the protective effect of Withaferin-A on DMBA-induced micronucleus frequency in the bone marrow of golden Syrian hamsters. Also, Withaferin-A maintained the status of buccal mucosa detoxication agents during DMBA-induced hamster buccal pouch carcinogenesis.
我们的目的是研究 Withaferin-A 对 7,12-二甲基苯并[a]蒽（DMBA）诱导的仓鼠颊囊癌变过程中骨髓微核频率和颊黏膜解毒剂的影响。通过在液体石蜡中涂抹 0.5%的 DMBA，每周三次，持续 14 周，在仓鼠颊囊中诱发口腔鳞状细胞癌。我们观察到涂抹 DMBA 的仓鼠出现了 100%的肿瘤形成。在荷瘤仓鼠中，骨髓微核多染红细胞（MnPCEs）频率升高，颊黏膜 II 期解毒剂减少。口服 Withaferin-A 可显著降低微核频率，并使涂抹 DMBA 的仓鼠恢复 II 期解毒剂的状态。因此，我们的研究证明了 Withaferin-A 对叙利亚金黄仓鼠骨髓中 DMBA 诱导的微核频率具有保护作用。此外，Withaferin-A 在 DMBA 诱导的仓鼠颊囊癌变过程中维持了颊黏膜解毒剂的状态。
