Department of Diagnosis and Surgery, São Paulo State University (UNESP - São José dos Campos), School of Dentistry, Brazil.
Int J Oral Maxillofac Implants. 2009 Nov-Dec;24(6):1074-82.
The aim of this study was to quantitatively evaluate and qualitatively describe autogenous bone graft healing with or without an expanded polytetrafluoroethylene (e-PTFE) membrane in ovariectomized rats.
Eighty Wistar rats, weighing approximately 300 g each, were used. A graft was obtained from the parietal bone and fixed to the sidewall of each animal's left mandibular ramus. The animals were randomly divided into four experimental groups (n = 20 in each group): group 1, sham operated and autogenous bone graft only; group 2, sham operated and autogenous bone graft covered by e-PTFE membrane; group 3, ovariectomized (OVX) and autogenous bone graft only; group 4, OVX and autogenous bone graft covered by e-PTFE membrane. The animals were sacrificed at five different time points: immediately after grafting or at 7, 21, 45, or 60 days after grafting. Histologic examination and morphometric measurement of the sections were performed, and values were submitted to statistical analyses.
Both groups (sham and OVX) experienced loss of the original graft volume when it was not covered by the membrane, whereas use of the membrane resulted in additional bone formation beyond the edges of the graft and under the membrane. Histologic analysis showed integration of the grafts in all animals, although a larger number of marrow spaces was found in OVX groups.
Association of bone graft with an e-PTFE membrane resulted in maintenance of its original volume as well as formation of new bone that filled the space under the membrane. Osteopenia did not influence bone graft repair, regardless of whether or not it was associated with e-PTFE membrane, but descriptive histologic analysis showed larger numbers of marrow spaces in the bone graft and receptor bed and formation of new bone in the OVX animals.
本研究旨在定量评估和定性描述去卵巢大鼠自体骨移植物在有无膨化聚四氟乙烯（e-PTFE）膜覆盖下的愈合情况。
使用 80 只体重约 300 克的 Wistar 大鼠。从顶骨获取移植物，并将其固定在每只动物左侧下颌支侧壁上。动物随机分为四个实验组（每组 20 只）：组 1，假手术且仅自体骨移植；组 2，假手术且 e-PTFE 膜覆盖的自体骨移植；组 3，去卵巢（OVX）且仅自体骨移植；组 4，OVX 且 e-PTFE 膜覆盖的自体骨移植。动物在移植后 5 个不同时间点（移植后即刻或 7、21、45 或 60 天）处死。对切片进行组织学检查和形态计量测量，并对测量值进行统计分析。
两组（假手术和 OVX）在未覆盖膜时均出现原始移植物体积丢失，而使用膜则导致移植物边缘和膜下有额外的骨形成。组织学分析显示所有动物的移植物均整合，尽管 OVX 组发现更多的骨髓腔。
自体骨移植物与 e-PTFE 膜联合使用可维持其原始体积，并形成填充膜下空间的新骨。骨质疏松症无论是否与 e-PTFE 膜联合使用，都不会影响骨移植物修复，但描述性组织学分析显示，在 OVX 动物中，骨移植物和受体床中有更多的骨髓腔，并且形成了新骨。
