The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK CB10 1SA.
Lab Chip. 2010 Mar 7;10(5):544-7. doi: 10.1039/b917560a. Epub 2009 Dec 15.
Technological advances and economic investment into DNA sequencing during this decade has provided the industry of genome sequencing with a suite of dedicated sequencing machines capable of rapidly generating vast quantities of sequence data. This next generation of equipment for DNA sequencing is freely available and is utilised more commonly; this has lead to the traditional bottle-neck in the sequencing pipeline transferring from the sequencing process, i.e. reading the bases on the older capillary based machines, to the upstream processes of sample preparation, i.e. creating the DNA libraries that are to be read. Essentially, advancement in sequencing technology is running faster than the equivalent for sample preparation technology and, without a remedy, we will no longer be able to provide samples quick enough to keep the sequencing machines running at full capacity.
在过去十年中，DNA 测序技术的进步和经济投入为基因组测序行业提供了一系列专用测序仪器，这些仪器能够快速生成大量的序列数据。这种新一代的 DNA 测序设备是免费提供的，并且应用得越来越普遍；这导致测序管道中的传统瓶颈从测序过程（即在较旧的毛细管基础机器上读取碱基）转移到了上游的样本制备过程（即创建要读取的 DNA 文库）。从本质上讲，测序技术的进步速度快于样本制备技术，而且如果不加以解决，我们将无法及时提供样本，以使测序仪器保持满负荷运行。
