Coimbra Sílvia, Costa Mário, Mendes Marta Adelina, Pereira Ana Marta, Pinto João, Pereira Luís Gustavo
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Edifício FC4 Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.
Sex Plant Reprod. 2010 Sep;23(3):199-205. doi: 10.1007/s00497-010-0136-x. Epub 2010 Feb 17.
The pollen specificity of the Arabidopsis arabinogalactan protein (AGP) genes AGP6 and AGP11 suggests that they are integral to pollen biogenesis, and their high percent of sequence similarity may indicate a potential for overlapping function. Arabidopsis agp6 agp11 double null mutants have been studied in our laboratory, and in the present work, we characterize the germination and growth of its pollen. When compared to wild type, mutant agp6 agp11 pollen displayed reduced germination and elongation, both in vivo and in vitro, and precocious germination inside the anthers, provided that sufficient moisture was available. This characteristic was not observed in wild type plants, even in water content conditions which for the mutant were sufficient for pollen germination. Therefore, an additional distinctive phenotypic trait of arabinogalactan proteins AGP6 and AGP11 may be to avert untimely germination of pollen. Such AGPs may control germination through water uptake, suggesting an important biological function of this gene family in pollen.
拟南芥阿拉伯半乳聚糖蛋白（AGP）基因AGP6和AGP11的花粉特异性表明它们是花粉生物发生所必需的，并且它们较高的序列相似百分比可能表明存在功能重叠的潜力。我们实验室已经对拟南芥agp6 agp11双缺失突变体进行了研究，在本研究中，我们对其花粉的萌发和生长进行了表征。与野生型相比，突变体agp6 agp11花粉在体内和体外的萌发和伸长均减少，并且在花药内出现早熟萌发，前提是有足够的水分。在野生型植物中未观察到这种特性，即使在对突变体而言足以支持花粉萌发的水分含量条件下也是如此。因此，阿拉伯半乳聚糖蛋白AGP6和AGP11的另一个独特表型特征可能是避免花粉过早萌发。此类AGP可能通过水分吸收来控制萌发，这表明该基因家族在花粉中具有重要的生物学功能。
