College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Vet Res Commun. 2010 Feb;34(2):143-52. doi: 10.1007/s11259-010-9340-2. Epub 2010 Feb 17.
Medullary bone is a unique tissue in the long bones cavities of lay hens, and plays an important role as a calcium reservoir for egg-shell formation. Medullary bone formation requires the synergistic action of estrogen and androgen on osteoblasts during the early stage of sexual maturity. The objective of the current study was to investigate the effects of 17beta-estradiol, testosterone, and the combination on the proliferation, alkaline phosphatase (ALP) activity, apoptosis, the cell cycle of chicken osteoblasts in vitro. The proliferation of osteoblasts was examined with the MTT assay. Apoptosis and the cell cycle were assessed with flow cytometry. Either 17beta-estradiol (200 pg ml(-1)) or testosterone (100 pg ml(-1)) or the combination (100 pg ml(-1) each) significantly enhanced osteoblast proliferation and ALP activity, accelerated the osteoblast cell cycle, and stimulated osteoblast DNA synthesis in a period of 24 h. 17beta-estradiol, used alone or with testosterone, inhibited chicken osteoblast apoptosis; However, testosterone alone induced cell apoptosis. In conclusion, 17beta-estradiol combined with testosterone promoted osteoblast proliferation and ALP activity, accelerated the osteoblast cell cycle, inhibited osteoblast apoptosis.
骨髓骨是产蛋母鸡长骨腔中的一种独特组织，作为蛋壳形成的钙库发挥着重要作用。骨髓骨的形成需要雌激素和雄激素在性成熟早期协同作用于成骨细胞。本研究的目的是研究 17β-雌二醇、睾酮及其组合对体外鸡成骨细胞增殖、碱性磷酸酶（ALP）活性、细胞凋亡和细胞周期的影响。用 MTT 法检测成骨细胞的增殖。用流式细胞术评估细胞凋亡和细胞周期。单独使用 17β-雌二醇（200 pg/ml）或睾酮（100 pg/ml）或联合使用（各 100 pg/ml）均可在 24 小时内显著促进成骨细胞增殖和 ALP 活性，加速成骨细胞周期，并刺激成骨细胞 DNA 合成。17β-雌二醇单独使用或与睾酮联合使用均可抑制鸡成骨细胞凋亡；然而，单独使用睾酮会诱导细胞凋亡。总之，17β-雌二醇与睾酮联合促进成骨细胞增殖和 ALP 活性，加速成骨细胞周期，抑制成骨细胞凋亡。
