Mikolajczuk Agnieszka, Przyk Elzbieta Perez, Geypens Benny, Berglund Michael, Taylor Philip
Joint Research Centre-European Commission, Institute for Reference Materials and Measurements, Geel, Belgium.
Isotopes Environ Health Stud. 2010 Mar;46(1):2-12. doi: 10.1080/10256010903356920.
Compound specific isotopic analysis (CSIA) can provide information about the origin of analysed compounds - in this case, polycyclic aromatic hydrocarbons (PAHs). In the study, PAHs were extracted from three dust samples: winter and summer filter dust and tunnel dust. The measurement was performed using the method validated in our laboratory using pure, solid compounds and EPA 610 reference assortment. CSIA required an appropriate clean-up method to avoid an unresolved complex in the gas chromatographic analysis usually found in the chromatography of PAHs. Extensive sample clean-up for this particular matrix was found to be necessary to obtain good gas chromatography-combustion-isotope ratio mass spectrometry analysis results. The sample purification method included two steps in which the sample is cleaned up and the aliphatic and aromatic hydrocarbons are separated. The concentration of PAHs in the measured samples was low; so a large volume injection technique (100 microl) was applied. The delta(VPDB)(13)C was measured with a final uncertainty smaller than 1 per thousand. Comparison of the delta(VPDB)(13)C signatures of PAHs extracted from different dust samples was feasible with this method and, doing so, significant differences were observed.
化合物特异性同位素分析（CSIA）能够提供有关被分析化合物来源的信息——在此案例中，指多环芳烃（PAHs）。在该研究中，多环芳烃是从三个灰尘样本中提取的：冬季和夏季的过滤灰尘以及隧道灰尘。测量是使用在我们实验室中针对纯固体化合物和EPA 610参考分类进行验证的方法来进行的。CSIA需要一种合适的净化方法，以避免在多环芳烃色谱分析中通常会出现的气相色谱分析中未解析的复杂情况。发现对于这种特定基质进行广泛的样品净化对于获得良好的气相色谱-燃烧-同位素比率质谱分析结果是必要的。样品净化方法包括两个步骤，在这两个步骤中对样品进行净化并分离脂肪族和芳香族烃类。所测样品中多环芳烃的浓度较低；因此采用了大体积进样技术（100微升）。测量的δ(VPDB)(13)C的最终不确定度小于千分之一。用此方法可以比较从不同灰尘样本中提取的多环芳烃的δ(VPDB)(13)C特征，并且这样做时，观察到了显著差异。
