Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an 710069, PR China.
Electrophoresis. 2010 Jan;31(4):630-3. doi: 10.1002/elps.200900463.
delta-Gluconolactone was covalently coupled with aminopropyl-derivatized capillary, creating hydrophilic brushes on the inner wall of the capillary. The hydrophilic coating provided suppression of EOF and minimized protein adsorption, resulting in the separation of basic proteins and DNA with efficiencies up to 450 000 plates/m. The intra- and inter-day repeatabilities of the coating referring to the migration times of the four tested proteins were satisfactory with RSD of no more than 1.1 and 1.8% (n=5), respectively. Two hundred consecutive runs were performed with negligible change in migration times and efficiency.
δ-葡萄糖酸内酯通过共价键与氨丙基衍生化的毛细管相连，在毛细管的内壁上形成亲水性刷。亲水性涂层提供了对EOF 的抑制作用，并最大限度地减少了蛋白质的吸附，从而实现了碱性蛋白质和 DNA 的分离，效率高达 450000 板/m。该涂层的日内和日间重复性（参考四种测试蛋白质的迁移时间）令人满意，相对标准偏差（RSD）分别不超过 1.1%和 1.8%（n=5）。连续进行 200 次运行，迁移时间和效率几乎没有变化。
