兔α-1-抗蛋白酶F编码cDNA的克隆与测序：六种哺乳动物α-1-抗蛋白酶的氨基酸序列比较

Cloning and sequencing of cDNA coding for rabbit alpha-1-antiproteinase F: amino acid sequence comparison of alpha-1-antiproteinases of six mammals.

作者信息

Saito A, Sinohara H

机构信息

Department of Biochemistry, School of Medicine, Kinki University, Osaka.

出版信息

J Biochem. 1991 Jan;109(1):158-62. doi: 10.1093/oxfordjournals.jbchem.a123338.

DOI:10.1093/oxfordjournals.jbchem.a123338

PMID:2016265

Abstract

Rabbit liver cDNA coding for alpha-1-antiproteinase F has been isolated and sequenced. The protein sequence deduced from the nucleotide sequence consists of a 24 amino acid signal peptide and 389 amino acids of the mature polypeptide. Rabbit alpha-1-antiproteinase F showed 74 and 64% homology to human alpha-1-antiproteinase at the nucleotide and amino acid levels, respectively, but the N-terminal five amino acids are lacking in the rabbit protein. The sequences of alpha-1-antiproteinase F of rabbit, human, baboon, sheep, rat, and mouse show about 40% identity, and the reactive site (Met-Ser) is conserved. On the other hand, variable regions are located in the second half to the C-terminal as well as in the N-terminal region.

摘要

编码α-1-抗蛋白酶F的兔肝脏cDNA已被分离并测序。从核苷酸序列推导的蛋白质序列由一个24个氨基酸的信号肽和389个氨基酸的成熟多肽组成。兔α-1-抗蛋白酶F在核苷酸和氨基酸水平上分别与人α-1-抗蛋白酶显示出74%和64%的同源性，但兔蛋白缺乏N端的五个氨基酸。兔、人、狒狒、绵羊、大鼠和小鼠的α-1-抗蛋白酶F序列显示约40%的同一性，并且活性位点（甲硫氨酸-丝氨酸）是保守的。另一方面，可变区位于C端后半部分以及N端区域。