Oderfeld-Nowak B, Zaremba M, Kwiatkowska-Patzer B, Lipkowski A W, Kurkowska-Jastrzebska I, Triaca V, Aloe L
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Arch Ital Biol. 2009 Dec;147(4):105-15.
Data have been provided from several studies that support the proposal that the adult oligodendrocyte progenitors migrate into the lesioned areas under conditions of experimental autoimmune encephalomyelitis (EAE). However, the routes of migration of these cells and the governing mechanisms are not clear. In the present studies, we have examined the effect of EAE upon activation of endogenous oligodendroglia progenitors and their spatial distribution in the spinal cord of Lewis rats using immunocytochemical procedures. Antibodies against the marker chondroitin sulfate proteoglycan NG2, are used for identification of oligodendroglia progenitors. We find that the activated elongated subpopulation of NG2 positive oligodendroglia progenitors of white matter is spatially associated with the radially-oriented astroglia during the acute phase of EAE. The latter re-expressed the phenotypic embryonic marker nestin while still expressing the mature astroglial marker GFAP. The elongated oligodendroglia progenitors express p75 receptor. In addition, colocalization of NG2 and p75 is observed also in ependymal neural cells of the central canal and the subventricular zone. This raises the possibility that the activated NG2+/p75+ parenchymal cell pool may also be recruited from multipotent neural cells of the germination areas. Our data suggest that, under EAE conditions, the radially oriented astroglia of juvenile phenotype may serve as scaffolding for migrating activated endogenous oligodendroglia progenitors just like radial glia provide a path for neuronal and oligodendroglia progenitor cells in embryonic stage. The expression of p75 receptor in oligodendroglia progenitors associated with radially oriented astroglia during EAE may implicate a role for NGF in the regulation of migration of oligodendroglia progenitors.
在实验性自身免疫性脑脊髓炎（EAE）条件下，成年少突胶质前体细胞会迁移至损伤区域。然而，这些细胞的迁移途径及调控机制尚不清楚。在本研究中，我们运用免疫细胞化学方法，检测了EAE对Lewis大鼠脊髓内源性少突胶质前体细胞激活及其空间分布的影响。针对硫酸软骨素蛋白聚糖NG2这一标志物的抗体，用于鉴定少突胶质前体细胞。我们发现，在EAE急性期，白质中被激活的细长型NG2阳性少突胶质前体细胞在空间上与呈放射状排列的星形胶质细胞相关。后者重新表达了表型胚胎标志物巢蛋白，同时仍表达成熟的星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）。细长型少突胶质前体细胞表达p75受体。此外，在中央管和脑室下区的室管膜神经细胞中也观察到NG2和p75的共定位。这增加了一种可能性，即被激活的NG2+/p75+实质细胞池也可能从生发区的多能神经细胞中募集而来。我们的数据表明，在EAE条件下，幼年表型的呈放射状排列的星形胶质细胞可能为迁移的被激活的内源性少突胶质前体细胞提供支架，就如同放射状胶质细胞在胚胎期为神经元和少突胶质前体细胞提供路径一样。EAE期间，与呈放射状排列的星形胶质细胞相关的少突胶质前体细胞中p75受体的表达可能意味着神经生长因子（NGF）在少突胶质前体细胞迁移调控中发挥作用。
