Shin Tae-kyun, Lee Yong-duk, Sim Ki-bum
Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University, Jeju, Korea.
J Vet Sci. 2003 Apr;4(1):9-13.
Intermediate filaments, including nestin and vimentin, are found in specific cell types in central nervous system (CNS) tissues, particularly immature glial cells and multipotent progenitor cells. In the present study, the expression patterns of nestin and vimentin in the spinal cords of rats with experimental autoimmune encephalomyelitis (EAE) and the response of cells containing filaments against acute autoimmune injury were examined by immunohistochemistry. Nestin immunostaining was only weakly detected in vascular endothelial cells but not in any cell types in the spinal cord in normal and adjuvant-immunized rats. At the peak stage of EAE, nestin-immunoreativity was recognized in some astrocytes in the gray matter and white matter. Vimentin was immunopositive in some astrocytes and macrophages in EAE lesions, while vimentin was normally detected in ependymal cells of central canals in the rat spinal cords.We postulate that normal animals may contain multipotent progenitor cells in the spinal cord parenchyma as well as in the subpial lesion and ependyma. Multipotent progenitor cells may activate to transform into necessary cells, including neurons, astrocytes or oligodendrocytes, depending on CNS needs. Appropriate control of progenitor cells in the injured CNS is an alternative choice for CNS remodeling.
中间丝，包括巢蛋白和波形蛋白，存在于中枢神经系统（CNS）组织的特定细胞类型中，特别是未成熟的神经胶质细胞和多能祖细胞。在本研究中，通过免疫组织化学检查了实验性自身免疫性脑脊髓炎（EAE）大鼠脊髓中巢蛋白和波形蛋白的表达模式以及含丝细胞对急性自身免疫损伤的反应。在正常和佐剂免疫的大鼠中，仅在血管内皮细胞中微弱检测到巢蛋白免疫染色，而在脊髓的任何细胞类型中均未检测到。在EAE的高峰期，在灰质和白质的一些星形胶质细胞中识别出巢蛋白免疫反应性。波形蛋白在EAE病变中的一些星形胶质细胞和巨噬细胞中呈免疫阳性，而波形蛋白通常在大鼠脊髓中央管的室管膜细胞中检测到。我们推测正常动物的脊髓实质、软膜下病变和室管膜中可能含有多能祖细胞。多能祖细胞可能会根据中枢神经系统的需要激活并转化为必要细胞，包括神经元、星形胶质细胞或少突胶质细胞。对受损中枢神经系统中的祖细胞进行适当控制是中枢神经系统重塑的另一种选择。
