Faculty of Agriculture, Iwate University, Morioka, Japan.
Anim Sci J. 2009 Feb;80(1):1-11. doi: 10.1111/j.1740-0929.2008.00589.x.
Studies on the neuroregulatory mechanisms on the secretion of anterior pituitary (AP) hormones in domestic animals are important because nearly all complex physiological and metabolic processes are regulated by the AP hormones. To examine them, this article considers in vivo approaches such as the techniques of intrahypothalamic injection, intracerebroventricular injection, push-pull perfusion, and microdialysis, which have been employed in our own research group for the study in cattle. Also, in vitro approaches such as bovine AP cell culture and the AP explants superfusion system are described. This article clarifies the potential of neuroendocrine study techniques in cattle.
对家畜前垂体（AP）激素分泌的神经调节机制的研究非常重要，因为几乎所有复杂的生理和代谢过程都受到 AP 激素的调节。为了研究这些机制，本文考虑了体内方法，如下丘脑内注射、脑室内注射、推挽灌流和微透析等技术，这些技术已被我们的研究小组用于牛的研究。此外，还描述了体外方法，如牛 AP 细胞培养和 AP 外植体灌流系统。本文阐明了神经内分泌研究技术在牛中的潜力。
