Laboratory of Animal Genetics and Breeding, Azabu University, Sagamihara, Japan.
Anim Sci J. 2009 Apr;80(2):176-86. doi: 10.1111/j.1740-0929.2008.00610.x.
Complementary DNA (cDNA) of prolactin (PRL) and vasoactive intestinal polypeptide (VIP) of the Java sparrow were cloned and sequenced. The proximal region of the PRL promoter was also identified. Java sparrow PRL was found to have 88.3, 88.3, and 89.1% sequence identity at the cDNA level to PRL of chicken, turkey, and duck, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of chicken (91.4%), turkey (88.9%) and duck (92.0%) PRL. Based on the cDNA sequence and genomic structure of the chicken PRL gene, the proximal promoter was characterized. Sequence analysis of the proximal region of Java sparrow PRL promoter revealed a high degree of similarity to that of chicken, turkey and duck PRL promoters. Moreover, cDNA of prepro-VIP was also cloned and sequenced. Java sparrow prepro-VIP shows high similarity to chicken and turkey prepro-VIP. However, the region upstream of the 5' untranslated region of Java sparrow prepro-VIP did not show similarity to that of chicken. These results suggest that the mechanisms, which regulate expression of the VIP gene, may be different between precocial and altricial birds, but expression of the PRL gene may be widely conserved in avian species.
已克隆和测序了爪哇麻雀催乳素 (PRL) 和血管活性肠肽 (VIP) 的 cDNA，并鉴定了 PRL 启动子的近端区域。发现爪哇麻雀 PRL 在 cDNA 水平上与鸡、火鸡和鸭的 PRL 分别具有 88.3、88.3 和 89.1%的序列同一性。预测的氨基酸序列与鸡（91.4%）、火鸡（88.9%）和鸭（92.0%）PRL 的可比区域具有总体相似性。基于鸡 PRL 基因的 cDNA 序列和基因组结构，对近端启动子进行了特征描述。爪哇麻雀 PRL 启动子近端区域的序列分析显示与鸡、火鸡和鸭 PRL 启动子具有高度相似性。此外，还克隆和测序了 prepro-VIP 的 cDNA。爪哇麻雀 prepro-VIP 与鸡和火鸡 prepro-VIP 具有高度相似性。然而，爪哇麻雀 prepro-VIP 的 5'非翻译区上游区域与鸡的没有相似性。这些结果表明，调节 VIP 基因表达的机制在早成性和晚成性鸟类之间可能不同，但 PRL 基因的表达在禽类中可能广泛保守。
