Koblas T, Zacharovová K, Berková Z, Leontovic I, Dovolilová E, Zámecník L, Saudek F
Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Folia Biol (Praha). 2009;55(6):224-32.
In our study we confirmed the potential of human umbilical cord blood cells to differentiate into insulin-producing cells following transplantation into immunocompromised mice. The average number of C-peptide-positive human cells per animal was 18 +/- 13 as assessed by immunofluorescence staining and fluorescence in situ hybridization specific for human ALU sequence. Differentiation into insulin-producing cells was further confirmed by reverse transcription-polymerase chain reaction specific for human insulin mRNA. Successful differentiation required sublethal irradiation of xenogeneic recipient at least at a dose of 3 Gy. However, transplantation of human umbilical cord blood cells did not improve hyperglycaemia in diabetic animals. The results of our study show that human umbilical cord blood may be considered as a potential source of stem cells for treatment of diabetes mellitus.
在我们的研究中，我们证实了将人脐带血细胞移植到免疫受损小鼠体内后，其具有分化为胰岛素产生细胞的潜力。通过免疫荧光染色和针对人ALU序列的荧光原位杂交评估，每只动物中C肽阳性人类细胞的平均数量为18±13。通过针对人胰岛素mRNA的逆转录-聚合酶链反应进一步证实了向胰岛素产生细胞的分化。成功的分化需要对异种受体进行至少3 Gy剂量的亚致死照射。然而，人脐带血细胞的移植并未改善糖尿病动物的高血糖症。我们的研究结果表明，人脐带血可被视为治疗糖尿病的潜在干细胞来源。
