Department of Antibiotics, Federal University of Pernambuco, 50.670-901-Recife-PE, Brazil.
Bioresour Technol. 2010 Jun;101(12):4725-8. doi: 10.1016/j.biortech.2010.01.091. Epub 2010 Feb 16.
During the fructose and polymerization to synthesize levan, smaller fructooligosaccharide (FOS) molecules are produced. FOS can also be obtained by levan hydrolysis. Three-phase partitioning (TPP) is a separation technique that has been used for polysaccharide precipitation and gathers t-butanol and ammonium sulphate to exclude the polymer from the aqueous solution. In this work TPP was tested to separate levan and FOS from aqueous solution. The FOS used was obtained from Zymomonas mobilis levan acid hydrolysis and fractionation with ethanol. The yield of low TPP fractions was higher than those obtained from the native levan. The F-90 exhibited a higher yield than other fractions. However, when applying the TPP technique to lightest fraction not precipitated by ethanol at 90% (F>90), the intermediate phase was not possible to be visualize. These results have potential application because they show that by using the levan TPP separation it is possible to separate low-molecular weight sugar.
在果糖和聚合合成果聚糖的过程中，会产生较小的低聚果糖（FOS）分子。FOS 也可以通过果聚糖水解获得。三相分配（TPP）是一种已被用于多糖沉淀的分离技术，它将叔丁醇和硫酸铵聚集起来，将聚合物从水溶液中排除。在这项工作中，TPP 被测试用于从水溶液中分离果聚糖和 FOS。所用的 FOS 是通过运动发酵单胞菌果聚糖酸水解和乙醇分级分离得到的。低 TPP 级分的产率高于天然果聚糖的产率。F-90 比其他级分的产率更高。然而，当将 TPP 技术应用于用 90%乙醇不能沉淀的最轻级分（F>90）时，中间相无法可视化。这些结果具有潜在的应用价值，因为它们表明，通过使用果聚糖 TPP 分离，可以分离低分子量的糖。
