Food Science College, Tibet Agriculture & Animal Husbandry University, Linzhi, 860000, China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, China.
Molecules. 2019 Jan 22;24(3):403. doi: 10.3390/molecules24030403.
Polysaccharides from the immunomodulatory medicinal mushroom (IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH₄)₂SO₄ 20% (); 11 mL -butanol; pH 8.0; temperature 30 °C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS' Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 μmol Trolox/g sample and 1040.5 μmol Fe/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent.
从免疫调节药用蘑菇（IOPS）中提取和纯化多糖，使用三相分配（TPP），这是一种高效，快速，安全和绿色的纯化技术。使用以下方案确定了最佳提取程序，可获得良好的 2.2％分离产率：固液比为 1 g 至 12 mL；（NH₄）₂SO₄ 质量分数为 20％（）；11 mL -丁醇；pH 值为 8.0；温度为 30°C；提取时间为 30 分钟。纯化的 IOPS 被证明是一种分子量为 40 kDa 的蛋白聚糖，由摩尔比为 2.0:3.5:1.0:1.5 的 d-半乳糖，d-葡萄糖，d-木糖和 d-甘露糖组成。纯化的 IOPS 在体外显示出很强的自由基清除能力，抗氧化活性和免疫活性。IOPS 的 Trolox 抗氧化等效能力和血浆的铁还原能力分别为 251.2 μmol Trolox/g 样品和 1040.5 μmol Fe/g 样品，其免疫调节行为的活性呈梯度依赖性。
