Izdebska Magdalena, Grzanka Alina, Ostrowski Maciej, Zuryń Agnieszka, Grzanka Dariusz
Department of Histology and Embryology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Bydgoszcz, Poland.
Folia Histochem Cytobiol. 2009 Jan;47(3):453-9. doi: 10.2478/v10042-009-0080-5.
Actin is one of the cytoskeletal proteins that take part in many cellular processes. The aim of this study was to show the influence of Trisenox (arsenic trioxide), on the cytoplasmic and nuclear F-actin organization. Arsenic trioxide is the proapoptotic factor. Together with increasing doses, it caused the increase in the number of cells undergoing apoptosis. Under arsenic trioxide treatment, cytoplasmic and nuclear F-actin (polymerized form of G-actin) was found reorganized. It was transformed into granulated structures. In cytometer studies fluorescence intensity of cytoplasmic F-actin after ATO treatment decreasing urgently in comparison to control. The obtained results may suggest the involvement of F-actin in apoptosis, especially in chromatin reorganization.
肌动蛋白是参与许多细胞过程的细胞骨架蛋白之一。本研究的目的是展示三氧化二砷（Trisenox）对细胞质和细胞核中F-肌动蛋白组织的影响。三氧化二砷是一种促凋亡因子。随着剂量增加，它导致凋亡细胞数量增加。在三氧化二砷处理下，发现细胞质和细胞核中的F-肌动蛋白（G-肌动蛋白的聚合形式）发生了重组。它转变为颗粒状结构。在细胞仪研究中，与对照组相比，三氧化二砷处理后细胞质F-肌动蛋白的荧光强度急剧下降。所得结果可能表明F-肌动蛋白参与了细胞凋亡，尤其是在染色质重组过程中。
