Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Cancer Biomark. 2010;6(1):11-20. doi: 10.3233/CBM-2009-0115.
Urinary bladder cancer is a major epidemiological problem that continues to grow each year. It opens avenues for investigative research for the identification of new disease markers and diagnostic techniques. In this pilot study, utility of non-invasive (1)H NMR spectroscopy has been evaluated for probing the metabolic perturbations occurring in non-muscle invasive urinary bladder cancer. (1)H NMR spectra of urine of bladder cancer patients and controls (healthy and urinary tract infection/bladder stone) (n = 103) were acquired at 400MHz. The non-overlapping resonances of citrate, dimethylamine, phenylalanine, taurine and hippurate were first identified and then quantitated by (1)H NMR spectra, with respect to an external reference sodium-3-trimethylsilylpropionate (TSP). The concentrations of these metabolites were then statistically analyzed. The cancer patients showed significant (p < 0.05) variations in concentration of hippurate and citrate as compared with healthy controls and benign controls. The significant elevation in concentration of taurine was observed in urine of bladder cancer patients, which was below the sensitivity limit of 400MHz in control cases. However, stages Ta, T1 and carcinoma in situ (CIS) cannot be differentiated on the basis of altered metabolite indices but their composition may reflect the biochemical alterations in metabolism of cancer cells.
膀胱癌是一个日益严重的主要流行病学问题。这为寻找新的疾病标志物和诊断技术开辟了研究途径。在这项初步研究中，评估了无创（1）H NMR 光谱用于探测非肌肉浸润性膀胱癌发生的代谢紊乱的效用。在 400MHz 下采集了膀胱癌患者和对照组（健康和尿路感染/膀胱结石）（n=103）的尿液的（1）H NMR 光谱。首先通过（1）H NMR 光谱鉴定并定量了柠檬酸、二甲胺、苯丙氨酸、牛磺酸和马尿酸的非重叠共振峰，相对于外部参考物 3-三甲基硅丙酸钠（TSP）。然后对这些代谢物的浓度进行了统计学分析。与健康对照组和良性对照组相比，癌症患者的马尿酸和柠檬酸浓度有显著（p < 0.05）变化。在膀胱癌患者的尿液中观察到牛磺酸浓度显著升高，但在对照组中，其浓度低于 400MHz 的灵敏度极限。然而，基于改变的代谢物指数无法区分 Ta、T1 和原位癌（CIS），但其组成可能反映了癌细胞代谢的生化变化。
