Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Org Biomol Chem. 2010 Mar 7;8(5):1142-8. doi: 10.1039/b919347b. Epub 2010 Jan 8.
Functionalised thiols presenting peptides found in the peptidoglycan of vancomycin-sensitive and -resistant bacteria were synthesised and used to form self-assembled monolayers (SAMs) on gold surfaces. This model bacterial cell-wall surface mimic was used to study binding interactions with vancomycin. Force spectroscopy, using the atomic force microscope (AFM), was used to investigate the specific rupture of interfacial vancomycin dimer complexes formed between pairs of vancomycin molecules bound to peptide-coated AFM probe and substrate surfaces. Clear adhesive contacts were observed between the vancomycin-sensitive peptide surfaces when vancomycin was present in solution, and the adhesion force demonstrated a clear dependence on antibiotic concentration.
合成了带有功能化巯基的肽，这些肽存在于万古霉素敏感和耐药细菌的肽聚糖中，并用于在金表面形成自组装单层（SAM）。这个模型细菌细胞壁表面模拟物被用于研究与万古霉素的结合相互作用。使用原子力显微镜（AFM）的力谱学用于研究在结合到肽涂覆的 AFM 探针和基底表面的万古霉素分子对之间形成的界面万古霉素二聚体复合物的特异性破裂。当溶液中存在万古霉素时，在万古霉素敏感的肽表面之间观察到明显的粘附有性接触，并且粘附力明显取决于抗生素浓度。
