Safavian D, Shore J S
Department of Biology, York University, 4700 Keele Street, Toronto, ON, M3J 1P3, Canada.
Sex Plant Reprod. 2010 Sep;23(3):225-37. doi: 10.1007/s00497-010-0135-y. Epub 2010 Feb 18.
We investigate the anatomy and fine structure of styles and pollen tubes of two distylous Turnera species, which possess a heteromorphic self-incompatibility system. We use fluorescence and transmission electron microscopy to provide the first description of the cellular aspects of pollen-pistil interactions and ultrastructural changes to pollen tubes during the self-incompatibility response of the morphs. No obvious ultrastructural differences occur between pistils and compatible pollen tubes. Conspicuous differences were, however, observed between incompatible pollen tubes of the morphs. Incompatible pollen tubes of the long-styled morph always appear to be intact, and pollen tube tips are often highly fluorescent when stained with aniline blue, a stain for callose. Swelling and loss of cristae of mitochondria, and circular rough endoplasmic reticulum, were observed for incompatible pollen tubes of the long-styled morph. For incompatible pollen tubes of the short-styled morph, the tube cell wall apex and plasma membrane often appear to be ruptured and no easily recognizable organelles, such as mitochondria, can be discerned. Our results clearly show ultrastructural differences between the morphs and support the hypothesis that different self-incompatibility mechanisms might operate between them.
我们研究了两种具有异型自交不亲和系统的二型花柱Turnera物种的花柱和花粉管的解剖结构及精细结构。我们使用荧光显微镜和透射电子显微镜，首次描述了花粉与雌蕊相互作用的细胞层面情况以及在不同形态的自交不亲和反应过程中花粉管的超微结构变化。雌蕊与亲和花粉管之间未观察到明显的超微结构差异。然而，在不同形态的不亲和花粉管之间观察到了显著差异。长花柱形态的不亲和花粉管似乎总是完整的，用苯胺蓝（一种用于染胼胝质的染料）染色时，花粉管尖端通常具有很强的荧光。观察到长花柱形态的不亲和花粉管有线粒体嵴肿胀和消失以及圆形粗面内质网的现象。对于短花柱形态的不亲和花粉管，管细胞壁顶端和质膜常常似乎破裂，并且无法辨别出易于识别的细胞器，如线粒体。我们的结果清楚地显示了不同形态之间的超微结构差异，并支持了它们之间可能存在不同自交不亲和机制的假说。
