Athanasiou A, Shore J S
Department of Biology, York University, Ontario, Canada.
Genetics. 1997 Jun;146(2):669-79. doi: 10.1093/genetics/146.2.669.
We used nondenaturing isoelectric focusing (IEF) in a survey of plants from 11 populations to identify style and pollen proteins unique to the short-styled morph of Turnera scabra, T. sulnitata and T. krapovickasii. Three protein bands [approximately isoelectric points (pls) 6.1, 6.3 and 6.5] were found only in styles and stigmas of short-styled plants while two bands (approximately pls 6.7 and 6.8, M(r) 56 and 59 kD) occur only in pollen of short-styled plants. Some of these bands appear very late in development, within 24 hr before flowering. Two isozyme loci were mapped to an 8.7 cM region spanning the distyly locus. Using these isozyme markers we identified progeny exhibiting recombination adjacent to the distyly locus. No recombinants between the distyly locus and the locus or loci controlling the presence of the short-styled morph-specific proteins were obtained. This suggest that the loci encoding these proteins are either extremely tightly linked to the distyly locus and in complete disequilibrium with the S allele or exhibit morph-limited expression. Crosses to a plant showing an unusual style protein phenotype demonstrated that an additional unlinked locus is required for full expression of the style proteins. The function of the morph-specific proteins is unknown.
我们运用非变性等电聚焦（IEF）技术，对来自11个种群的植物进行了调查，以鉴定糙叶特纳草、苏氏特纳草和克拉波维茨氏特纳草短花柱形态所特有的花柱和花粉蛋白。仅在短花柱植物的花柱和柱头上发现了三条蛋白带[等电点（pI）约为6.1、6.3和6.5]，而仅在短花柱植物的花粉中出现了两条带（pI约为6.7和6.8，分子量56和59kD）。其中一些条带在发育后期才出现，即在开花前24小时内。两个同工酶基因座被定位到一个跨越花柱异长基因座的8.7厘摩区域。利用这些同工酶标记，我们鉴定出在花柱异长基因座附近发生重组的后代。在花柱异长基因座与控制短花柱形态特异性蛋白存在的一个或多个基因座之间未获得重组体。这表明编码这些蛋白的基因座要么与花柱异长基因座紧密连锁，且与S等位基因完全不平衡，要么表现出形态限制表达。与一株表现出异常花柱蛋白表型的植物杂交表明，花柱蛋白的完全表达需要一个额外的不连锁基因座。形态特异性蛋白的功能尚不清楚。
