College of Chemistry, Jilin University, Changchun, PR China.
Electrophoresis. 2010 Mar;31(6):1090-6. doi: 10.1002/elps.200900423.
The low molecular weight proteins can provide a lot of valuable information of biomarkers. To study these proteins, the high abundance and high molecular weight proteins must be removed prior to analysis. In this work, a simple and inexpensive disc SDS-PAGE to extract low molecular weight proteins from human serum and cutoff proteins larger than 30 kDa was developed. Some experimental conditions were examined. The experimental results obtained by plate SDS-PAGE and MALDI-TOF MS showed that the molecular weight of extracted proteins was about in the range from 0.3 to 28 kDa. Some experiments, including precipitation of proteins in organic solvents, SPE and cytochrome C test, were carried out and the experimental results demonstrated successful recovery of proteins/peptides with molecular weight from several hundreds of dalton to about 30 kDa. The experimental results obtained by plate SDS-PAGE indicated the repeatability was satisfactory.
低分子量蛋白质可以提供大量有价值的生物标志物信息。为了研究这些蛋白质，必须在分析之前去除高丰度和高分子量的蛋白质。在这项工作中，开发了一种简单且廉价的圆盘 SDS-PAGE，用于从人血清中提取低分子量蛋白质，并截留大于 30 kDa 的蛋白质。研究了一些实验条件。通过平板 SDS-PAGE 和 MALDI-TOF MS 获得的实验结果表明，提取的蛋白质的分子量约为 0.3 至 28 kDa。进行了一些实验，包括有机溶剂沉淀蛋白质、SPE 和细胞色素 C 测试，实验结果表明成功回收了分子量从几百道尔顿到约 30 kDa 的蛋白质/肽。平板 SDS-PAGE 的实验结果表明重复性令人满意。
