Schön Jennifer, Blottner Steffen, Gabler Christoph, Fickel Jörns
Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Growth Factors. 2010 Jun;28(3):202-10. doi: 10.3109/08977191003587668.
Vascular endothelial growth factor A (VEGFA) influences spermatogenesis, but its impact on seasonally regulated sperm production is still not fully understood. Thus, we investigated both expression levels and localisation of VEGFA and its receptors VEGFR1 and 2 in roe buck testis via real-time reverse transcription polymerase chain reaction and immunohistochemistry in relation to seasonal changes in the cellular composition of the testis. VEGFA was expressed by interstitial cells while its receptors were found on endothelial and perivascular cells. Inside the tubules, VEGFA was located in spermatogonia and spermatocytes, VEGFR1 was present on elongating spermatids and VEGFR2 on Sertoli cells. VEGFR1 mRNA was expressed tenfold lower than VEGFR2 and VEGF mRNAs. Relative VEGF and VEGFR2 expression (divided by the number of VEGFA and VEGFR2 expressing cells) showed an increase towards the rut (July/August) and a decrease thereafter. The results suggest involvement of VEGFA in the adjustment of vascular permeability as well as in spermiogenesis and the proliferation of spermatogonia.
血管内皮生长因子A（VEGFA）影响精子发生，但其对季节性调控的精子生成的影响仍未完全了解。因此，我们通过实时逆转录聚合酶链反应和免疫组织化学，研究了狍子睾丸中VEGFA及其受体VEGFR1和2的表达水平和定位，以及睾丸细胞组成的季节性变化。VEGFA由间质细胞表达，而其受体在内皮细胞和血管周围细胞上被发现。在曲细精管内，VEGFA位于精原细胞和精母细胞中，VEGFR1存在于伸长的精子细胞上，VEGFR2存在于支持细胞上。VEGFR1 mRNA的表达比VEGFR2和VEGF mRNA低十倍。相对VEGF和VEGFR2表达（除以表达VEGFA和VEGFR2的细胞数量）在发情期（7月/8月）增加，此后减少。结果表明VEGFA参与血管通透性的调节以及精子发生和精原细胞的增殖。
