Wise G E, Fan W
Department of Anatomy and Cell Biology, Texas College of Osteopathic Medicine, Fort Worth 76107.
J Oral Pathol Med. 1991 Feb;20(2):74-80. doi: 10.1111/j.1600-0714.1991.tb00893.x.
A prominent cellular event in tooth eruption is the influx of mononuclear cells (monocytes?) into the dental follicle at the onset of eruption. In the mandibular first molar of the rat, this influx of cells reaches its peak at three days postnatally. Because transforming growth factor-beta 1 (TGF-beta 1) is chemotactic for monocytes, we used immunocytochemical methods to determine its localization in the rat molar during postnatal days 1-4 and day 7. The results indicate that TGF-beta 1 displays both a spatial and temporal localization in the rat molar. It is present in the stellate reticulum (SR) on days 1 and 2 postnatally but is absent in all the subsequent days examined. None of the other soft tissue layers-ameloblasts, stratum intermedium or dental follicle--immunostain for TGF-beta 1. This localization of TGF-beta 1 in the SR at a time that just precedes that influx of monocytes into the dental follicle, coupled with the fact that fenestrated capillaries are abundant in the follicle adjacent to the SR, suggests that TGF-beta 1 could play a role in attracting monocytes from the peripheral blood into the follicle.
牙齿萌出过程中一个显著的细胞事件是在萌出开始时单核细胞（单核细胞？）流入牙囊。在大鼠下颌第一磨牙中，这种细胞流入在出生后三天达到峰值。由于转化生长因子-β1（TGF-β1）对单核细胞具有趋化作用，我们使用免疫细胞化学方法来确定其在出生后1至4天和第7天大鼠磨牙中的定位。结果表明，TGF-β1在大鼠磨牙中呈现出空间和时间上的定位。它在出生后第1天和第2天存在于星网状层（SR）中，但在随后检查的所有天数中均不存在。其他软组织层——成釉细胞、中间层或牙囊——均未检测到TGF-β1的免疫染色。TGF-β1在单核细胞流入牙囊之前的时间在SR中的这种定位，再加上与SR相邻的牙囊中存在丰富的有孔毛细血管，表明TGF-β1可能在将外周血中的单核细胞吸引到牙囊中发挥作用。
