Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China.
J Invertebr Pathol. 2010 Jun;104(2):90-6. doi: 10.1016/j.jip.2010.02.003. Epub 2010 Feb 16.
A field population (SZ) of Plutella xylostella, collected from the cabbage field in Shenzhen, Guangdong Province of China in 2002, showed 2.3-fold resistance to Cry1Aa, 110-fold to Cry1Ab, 30-fold to Cry1Ac, 2.1-fold to Cry1F, 5.3-fold to Cry2Aa and 6-fold resistance to Bacillus thuringiensis var. kurstaki (Btk) compared with a susceptible strain (ROTH). The SZBT strain was derived from the SZ population through 20 generations of selection with activated Cry1Ac in the laboratory. While the SZBT strain developed 1200-fold resistance to Cry1Ac after selection, resistance to Cry1Aa, Cry1Ab, Cry1F, and Btk increased to 31-, 1900-,>33- and 17-fold compared with the ROTH strain. However, little or no cross-resistance was detected to Cry1B, Cry1C and Cry2Aa in the SZBT strain. Genetic cross analyses between the SZBT and ROTH strains revealed that Cry1Ac-resistance in the SZBT strain was controlled by a single, autosomal, incompletely recessive gene. Binding studies with (125)I-labeled Cry1Ac showed that the brush border membrane vesicles (BBMVs) of midguts from the resistant SZBT insects had lost binding to Cry1Ac. Allelic complementation tests demonstrated that the major Bt resistance locus in the SZBT strain was same as that in the Cry1Ac-R strain which has "mode 1" resistance to Bt. An F(1) screen of 120 single-pair families between the SZBT strain and three field populations collected in 2008 was carried out. Based on this approach, the estimated frequencies of Cry1Ac-resistance alleles were 0.156 in the Yuxi population from Yunnan province, and 0.375 and 0.472 respectively in the Guangzhou and Huizhou populations from Guangdong province.
从中国广东省深圳市的白菜田中采集的 2002 年田间种群（SZ）对 Cry1Aa 的抗性增加了 2.3 倍，对 Cry1Ab 的抗性增加了 110 倍，对 Cry1Ac 的抗性增加了 30 倍，对 Cry1F 的抗性增加了 2.1 倍，对 Cry2Aa 的抗性增加了 5.3 倍，对苏云金芽孢杆菌 var. kurstaki（Btk）的抗性增加了 6 倍，与敏感品系（ROTH）相比。SZBT 菌株是通过在实验室中用激活的 Cry1Ac 对 SZ 种群进行 20 代选择而衍生出来的。虽然 SZBT 菌株在选择后对 Cry1Ac 的抗性增加了 1200 倍，但与 ROTH 菌株相比，对 Cry1Aa、Cry1Ab、Cry1F 和 Btk 的抗性分别增加到 31 倍、1900 倍、>33 倍和 17 倍。然而，在 SZBT 菌株中几乎没有或没有检测到对 Cry1B、Cry1C 和 Cry2Aa 的交叉抗性。SZBT 和 ROTH 菌株之间的遗传杂交分析表明，SZBT 菌株对 Cry1Ac 的抗性由一个单一的、常染色体、不完全隐性基因控制。用（125）I 标记的 Cry1Ac 进行的结合研究表明，抗性 SZBT 昆虫的中肠刷状缘膜泡（BBMVs）失去了与 Cry1Ac 的结合。等位基因互补测试表明，SZBT 菌株中的主要 Bt 抗性基因座与 Cry1Ac-R 菌株相同，该菌株对 Bt 具有“模式 1”抗性。在 SZBT 菌株和 2008 年收集的三个田间种群之间进行了 120 个单对家系的 F(1)筛选。基于这种方法，在云南省玉溪市种群中 Cry1Ac 抗性等位基因的估计频率为 0.156，在广东省广州市和惠州市种群中分别为 0.375 和 0.472。
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