二苯并噻吩（DBT）短期暴露后虹鳟鱼（Oncorhynchus mykiss）肝脏和鳃中的 CYP1A 表达。
CYP1A expression in liver and gills of rainbow trout (Oncorhynchus mykiss) after short-term exposure to dibenzothiophene (DBT).
机构信息
Department of Environmental Biotechnology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-719 Olsztyn, Poland.
出版信息
Chemosphere. 2010 Mar;79(1):110-2. doi: 10.1016/j.chemosphere.2010.01.063. Epub 2010 Feb 18.
Dibenzothiophene (DBT), a common component of crude oil, is a widespread environmental pollutant of known adverse effects to aquatic vertebrates. However, the molecular mechanism by which DBT exerts its effects still remains unknown. Our goal for this study was to examine DBT effects on CYP1A expression in liver and gills of rainbow trout after short-term exposure. Juvenile trout individuals were injected intraperitoneally with two doses of DBT (10 or 50mgkg(-1)) and were kept in tanks for 8 and 24h (T=14 degrees C), then their gene expression levels were evaluated by Real-Time qPCR and Western-blot analysis. Treatment with DBT at either dose decreased CYP1A mRNA levels through the exposure period, which resulted in the final decrease of CYP1A protein levels in liver and gills on the end of experiment (24h). Thus, our results showing significant depletion of CYP1A molecules in metabolic tissues upon DBT treatment correlate with those previous reports that indicate a role of DBT in reducing CYP1A activity in fish.
二苯并噻吩（DBT）是原油的常见成分，是一种广泛存在的环境污染物，已知对水生脊椎动物有不良影响。然而，DBT 发挥作用的分子机制尚不清楚。我们这项研究的目的是研究 DBT 对短期暴露后虹鳟鱼肝脏和鳃中 CYP1A 表达的影响。将幼年鳟鱼经腹腔注射两种剂量的 DBT（10 或 50mgkg(-1))，并在水箱中保持 8 和 24 小时（T=14 摄氏度），然后通过实时 qPCR 和 Western-blot 分析评估其基因表达水平。在暴露期间，两种剂量的 DBT 处理均降低了 CYP1A 的 mRNA 水平，最终导致实验结束时（24 小时）肝脏和鳃中 CYP1A 蛋白水平下降。因此，我们的结果表明，DBT 处理后代谢组织中 CYP1A 分子大量耗竭，与先前表明 DBT 在降低鱼类 CYP1A 活性中的作用的报告结果一致。
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