INRA, UR1037, SCRIBE, IFR140, Biogenouest, F-35000, Rennes, France.
Comp Biochem Physiol A Mol Integr Physiol. 2010 Jun;156(2):211-7. doi: 10.1016/j.cbpa.2010.02.002. Epub 2010 Feb 16.
During the transfer of rainbow trout from freshwater to seawater, the gills have to switch from an ion-absorption epithelium to an ion-secretion epithelium in order to maintain equilibrium of their hydromineral balance. After a change to ambient salinity, several gill modifications have already been demonstrated, including ion transporters. In order to identify new branchial mechanisms implicated in seawater acclimation, we carried out an extensive analysis of gene expression in gills using microarray technology. This strategy allowed us to show that CYP1A gene expression was up-regulated in the gills after salinity transfer. This increase was confirmed by real-time reverse transcription PCR. Furthermore, measurements of CYP1A enzyme activity (EROD) showed a significant increase after transfer to seawater. Immunohistochemistry analysis in the gills revealed that cells with a higher expression of CYP1A protein were principally pillar cells and those in the primary lamellae not in contact with the external medium. The results of this study suggest for the first time that CYP1A may be implicated in the seawater acclimation of the gills of rainbow trout.
在虹鳟鱼从淡水转移到海水中的过程中，其鳃必须从离子吸收上皮转变为离子分泌上皮，以维持其水盐平衡。在环境盐度发生变化后，已经证实了几种鳃的改变，包括离子转运体。为了确定参与海水适应的新鳃机制，我们使用微阵列技术对鳃中的基因表达进行了广泛分析。该策略使我们能够表明 CYP1A 基因在盐度转移后在鳃中被上调。通过实时逆转录 PCR 进行了确认。此外，在转移到海水中后，CYP1A 酶活性（EROD）的测量显示出明显增加。在鳃中的免疫组织化学分析表明，CYP1A 蛋白表达较高的细胞主要是支柱细胞，而那些在初级薄片中不与外部介质接触的细胞也是如此。这项研究的结果首次表明，CYP1A 可能参与了虹鳟鱼鳃的海水适应。
